Western Blot (WB)
(Western blot analysis of Neuroglobin expression in U251 (A), mouse liver (B), mouse brain (C), rat liver (D), rat brain (E) whole cell lysates.)
Rabbit anti-Human Neuroglobin Polyclonal Antibody | anti-NGB antibody
Anti-Neuroglobin Antibody
Immunofluorescence/Immunocytochemistry: 1/10 - 1/100
Upon delivery aliquot and store at -20°C for one year.
Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of Neuroglobin expression in U251 (A), mouse liver (B), mouse brain (C), rat liver (D), rat brain (E) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Neuroglobin expression in U251 (A), mouse liver (B), mouse brain (C), rat liver (D), rat brain (E) whole cell lysates.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Neuroglobin staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Neuroglobin staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq, Jul 2008]
Uniprot Description
NGB: Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis. Belongs to the globin family.
Chromosomal Location of Human Ortholog: 14q24.3
Molecular Function: oxygen transporter activity; protein binding
Research Articles on NGB
Similar Products
Product Notes
The NGB ngb (Catalog #AAA8247528) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Neuroglobin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Neuroglobin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: 1/500 - 1/2000 Immunofluorescence/Immunocytochemistry: 1/10 - 1/100. Researchers should empirically determine the suitability of the NGB ngb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuroglobin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
