GDF15 active protein

GDF15

Cat. Num. AAA553697

• Gene Names: GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
• Applications: SDS-Page
• Purity: >95%, as determined by SDS-PAGE and HPLC.
• Synonyms: GDF15; GDF15 active protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC.
• Form/Format: Lyophilized; PBS, pH 7.2
• Sequence: MTQGENHPSP NFNQYVRDQG AMTDQLSRRQ IREYQLYSRT SGKHVQVTGR RISATAEDGN KFAKLIVETD TFGSRVRIKG AESEKYICMN KRGKLIGKPS GKSKDCVFTE IVLENNYTAF QNARHEGWFM AFTRQGRPRQ ASRSRQNQRE AHFIKRLYQG QLPFPNHAEK QKQFEFVGSA PTRRTKRTRR PQPLT
• Sequence Length: 308

• Applicable Applications for GDF15 active protein: SDS-PAGE
• Application Notes: Recombinant GDF15 migrates as an approximately 21 kDa protein under reducing conditions in SDS-PAGE.
• Species: Human
• Source Note: Optimized DNA sequence encoding Human GDF15 mature chain was expressed in ECOLI. Recombinant Human Growth/differentiation factor 15 encodes amino acid residues 195-308.
• Biological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is = 0.5 ng/ml
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 153792495
• NCBI GeneID: 9518
• NCBI Accession #: NP_004855.2
• NCBI GenBank Nucleotide #: NM_004864.4
• UniProt Accession #: Q99988; O14629; P78360; Q9BWA0; Q9NRT0
• Molecular Weight: 34,140 Da
• NCBI Official Full Name: growth/differentiation factor 15 preproprotein
• NCBI Official Synonym Full Names: growth differentiation factor 15
• NCBI Official Symbol: GDF15
• NCBI Official Synonym Symbols: PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
• NCBI Protein Information: growth/differentiation factor 15
• UniProt Protein Name: Growth/differentiation factor 15
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF15
• UniProt Synonym Gene Names: NAG-1; NRG-1

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The protein is expressed in a broad range of cell types, acts as a pleiotropic cytokine and is involved in the stress response program of cells after cellular injury. Increased protein levels are associated with disease states such as tissue hypoxia, inflammation, acute injury and oxidative stress. [provided by RefSeq, Aug 2016]

Uniprot Description

Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses (PubMed:28953886, PubMed:28846097, PubMed:28846098, PubMed:28846099, PubMed:23468844, PubMed:29046435). Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem (PubMed:28953886, PubMed:28846097, PubMed:28846098, PubMed:28846099). It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which contitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions (PubMed:28953886). On hepatocytes, inhibits growth hormone signaling ().