Flt3 Ligand Active Protein | FLT3LG active protein

Flt3 Ligand, Recombinant, Mouse (Fms-like Tyrosine Kinase 3 Ligand, flk2 Ligand)

Cat. Num. AAA638200

• Gene Names: FLT3LG; FL; FLT3L
• Purity: Highly Purified; > 98% by SDS-PAGE and HPLC
• Synonyms: Flt3 Ligand; Recombinant; Mouse (Fms-like Tyrosine Kinase 3 Ligand; flk2 Ligand); FLT3LG active protein

• Host: E Coli
• Purity/Purification: Highly Purified; > 98% by SDS-PAGE and HPLC
• Form/Format: Supplied as a lyophilized powder without additives. The protein may appear as a haze or film which is difficult to see at the bottom of the vial.

• Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human AML5 cells is 1ng/ml corresponding to a specific activity of 1x10e6 units/mg. Flt3-ligand is fully biologically active when compared to standards.
• Country of Origin: USA
• Reconstitution: Reconstitute in sterile dH2O to a concentration of 0.1-1mg/ml. Further dilutions should be made into buffer containing carrier protein or medium containing serum.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. For long-term storage, may be stored at -20 degree C. Reconstituted product can be stored at 4 degree C for 1 week. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for FLT3LG active protein

Flt3 Ligand (flk2 ligand), is a growth factor that regulates proliferation of early hematopoietic cells. Flt3 Ligand binds to cells expressing the tryosine kinase receptor Flt3. Flt3 Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt 3 Ligand exerts no activity on mast cells. Multiple isoforms of Flt 3 Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane glycoprotein (209aa). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform. Recombinant murine Flt3-Ligand is a soluble 18.6kD protein consisting of 163 amino acid residues.

Product Categories/Family for FLT3LG active protein

Molecular Biology; MB-Tyrosine Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 38455416
• NCBI GeneID: 2323
• NCBI Accession #: NP_001450.2
• NCBI GenBank Nucleotide #: NM_001459.3
• UniProt Accession #: P49771; A0AVC2; B9EGH2
• Molecular Weight: 17.6kD
• NCBI Official Full Name: fms-related tyrosine kinase 3 ligand isoform 1
• NCBI Official Synonym Full Names: fms-related tyrosine kinase 3 ligand
• NCBI Official Symbol: FLT3LG
• NCBI Official Synonym Symbols: FL; FLT3L
• NCBI Protein Information: fms-related tyrosine kinase 3 ligand; flt3 ligand
• UniProt Protein Name: Fms-related tyrosine kinase 3 ligand
• Protein Family: Fms-related tyrosine kinase
• UniProt Gene Name: FLT3LG
• UniProt Synonym Gene Names: Flt3 ligand; Flt3L
• UniProt Entry Name: FLT3L_HUMAN

NCBI Description

Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 (see MIM 186910)-positive classical DCs and their CD103 (ITGAE; MIM 604682)-positive tissue counterparts (summary by Sathaliyawala et al., 2010 [PubMed 20933441]).[supplied by OMIM, Jan 2011]

Uniprot Description

FLT3LG: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: extracellular space; membrane; integral to membrane

Molecular Function: protein homodimerization activity; cytokine activity; receptor tyrosine kinase binding; receptor binding

Biological Process: positive regulation of natural killer cell differentiation; regulation of myeloid dendritic cell activation; lymphocyte differentiation; embryonic hemopoiesis; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; signal transduction; positive regulation of myoblast differentiation