FGF18 active protein

FGF18

Cat. Num. AAA553635

• Gene Names: FGF18; ZFGF5; FGF-18
• Applications: SDS-Page
• Purity: >95%, as determined by SDS-PAGE and HPLC.
• Synonyms: FGF18; FGF18 active protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC.
• Form/Format: Lyophilized; PBS, pH 7.2
• Sequence: AEENVDFRI HVENQTRARD DVSRKQLRLY QLYSRTSGKH IQVLGRRISA RGEDGDKYAQ LLVETDTFGS QVRIKGKETE FYLCMNRKGK LVGKPDGTSK ECVFIEKVLE NNYTALMSAK YSGWYVGFTK KGRPRKGPKT RENQQDVHFM KRYPKGQPEL QKPFKYTTVT KRSR
• Sequence Length: 207

• Applicable Applications for FGF18 active protein: SDS-PAGE
• Application Notes: Recombinant FGF18 migrates as an approximately 19 kDa protein under reducing conditions in SDS-PAGE.
• Species: Human
• Source Note: Optimized DNA sequence encoding Human FGF18 mature chain including a C-terminal 6xHis tag was expressed in ECOLI. Recombinant Human Fibroblast growth factor 18 encodes amino acid residues 28-207.
• Biological Activity: Determined by thymidine uptake by BaF3 cells expressing FGF-receptors is = 0.5 ng/ml, corresponding to a specific activity of = 2 x 10^6 units/mg.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug(1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C. Upon reconstitution, this product can be stored in working aliquots at 2 degree C - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 4503695
• NCBI GeneID: 8817
• NCBI Accession #: NP_003853.1
• NCBI GenBank Nucleotide #: NM_003862.3
• UniProt Accession #: O76093; Q6UWF1; D3DQL7
• Molecular Weight: 23,989 Da
• NCBI Official Full Name: fibroblast growth factor 18
• NCBI Official Synonym Full Names: fibroblast growth factor 18
• NCBI Official Symbol: FGF18
• NCBI Official Synonym Symbols: ZFGF5; FGF-18
• NCBI Protein Information: fibroblast growth factor 18
• UniProt Protein Name: Fibroblast growth factor 18
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF18
• UniProt Synonym Gene Names: FGF-18

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation.