FGF17 active protein

FGF17 Protein, Human, Recombinant

Cat. Num. AAA8121151

• Gene Names: FGF17; HH20; FGF-13; FGF-17
• Purity: >95% as determined by SDS-PAGE
• Synonyms: FGF17; FGF17 Protein; Human; Recombinant; Human FGF17 Protein; FGF-13 Protein; FGF-17 Protein; HH20 Protein; fibroblast growth factor 17; FGF17 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 1M NaCl, pH8.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Thr23-Thr216

• Species: Human
• Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.6-3ug/mL.
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the mature form of human FGF17 (O60258-1)(Thr23-Thr216) was expressed, with a N-terminal Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.6-3 ug/mL.)

Related Product Information for FGF17 active protein

Background: FGF-13, also known as FGF17, belongs to the fibroblast growth factor (FGF) family. Members of this family show broad mitogenic and cell survival activities, and play a role in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-13 is preferentially expressed in the embryonic brain. It interacts with FGFR3 and FGFR4. FGF-13 plays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. It is also required for normal brain development.

References

Polnaszek N, et al. (2004) FGF17 is an autocrine prostatic epithelial growth factor and is upregulated in benign prostatic hyperplasia. Prostate. 60(1):18-24.Xu J, et al. (2000) Temporal and spatial gradients of Fgf8 and Fgf17 regulate proliferation and differentiation of midline cerebellar structures. Development. 127(9):1833-43.Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.

NCBI and Uniprot Product Information

• NCBI GI #: 4503693
• NCBI GeneID: 8822
• NCBI Accession #: NP_003858.1
• NCBI GenBank Nucleotide #: NP_003858.1
• UniProt Accession #: O60258
• NCBI Official Full Name: fibroblast growth factor 17 isoform 1
• NCBI Official Synonym Full Names: fibroblast growth factor 17
• NCBI Official Symbol: FGF17
• NCBI Official Synonym Symbols: HH20; FGF-13; FGF-17
• NCBI Protein Information: fibroblast growth factor 17
• UniProt Protein Name: Fibroblast growth factor 17
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF17
• UniProt Synonym Gene Names: FGF-17
• UniProt Entry Name: FGF17_HUMAN

NCBI Description

This gene encodes a member of the fibroblast growth factor (FGF) family. Member of the FGF family possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is expressed during embryogenesis and in the adult cerebellum and cortex and may be essential for vascular growth and normal brain development. Mutations in this gene are the cause of hypogonadotropic hypogonadism 20 with or without anosmia. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

FGF17: Plays an important role in the regulation of embryonic development and as signaling molecule in the induction and patterning of the embryonic brain. Required for normal brain development. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; type 2 fibroblast growth factor receptor binding; type 1 fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; nervous system development; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; cell-cell signaling; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; signal transduction

Disease: Hypogonadotropic Hypogonadism 20 With Or Without Anosmia