FGF-18 active protein

FGF-18, mouse

Cat. Num. AAA546416

• Gene Names: FGF18; ZFGF5; FGF-18
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Synonyms: FGF-18; mouse; FGF-18 active protein

• Host: E. coli
• Purity/Purification: >95% by SDS-PAGE and HPLC analyses.
• Form/Format: Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl.
• Sequence: EENVDFRIHV ENQTRARDDV VSRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQAELQK PFKYTTVTKR SRRIRPTHPG

• Biological Activity: Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10⁶ IU/mg.
• Physical Appearance: Sterile filtered white lyophilized (freeze-dried) powder.
• Endotoxin: Less than 1 EU/ug of rMuFGF-18 as determined by LAL method.
• Reconsitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.
• Preparation and Storage: The lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferrably dessicated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into -20°C to -70°C. Avoid repeated freeze-thaw cycles.

Related Product Information for FGF-18 active protein

Murine FGF-18 is encoded by the FGF18 gene. By phylogenetic analysis and gene location analysis, FGF-18 is divided into FGF-8 subfamily which has three members FGF-8, FGF-17 and FGF-18. Using FGF knockout mice model, the numbers of this subfamily were testified that have crucial roles of in embryo development. FGF-18–/– mice have decreased expression of osteogenic markers and delayed long-bone ossification. FGF-18 has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. In addition, it also has significant roles in lung development and has an anabolic effect on cartilage formation.

NCBI and Uniprot Product Information

• NCBI GI #: 4503695
• NCBI GeneID: 8817
• NCBI Accession #: NP_003853.1
• NCBI GenBank Nucleotide #: NM_003862.2
• Molecular Weight: Approximately 21.0 kDa, a single non-glycosylated polypeptide chain containing 180 amino acids.
• NCBI Official Full Name: fibroblast growth factor 18
• NCBI Official Synonym Full Names: fibroblast growth factor 18
• NCBI Official Symbol: FGF18
• NCBI Official Synonym Symbols: ZFGF5; FGF-18
• NCBI Protein Information: fibroblast growth factor 18
• UniProt Protein Name: Fibroblast growth factor 18
• UniProt Gene Name: FGF18
• UniProt Synonym Gene Names: FGF-18
• UniProt Entry Name: FGF18_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF18: Plays an important role in the regulation of cell proliferation, cell differentiation and cell migration. Required for normal ossification and bone development. Stimulates hepatic and intestinal proliferation. Belongs to the heparin-binding growth factors family.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: extracellular region; extracellular space; nucleolus

Molecular Function: 1-phosphatidylinositol-3-kinase activity; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; type 1 fibroblast growth factor receptor binding; type 2 fibroblast growth factor receptor binding

Biological Process: anatomical structure morphogenesis; angiogenesis; cell-cell signaling; chondrocyte development; endochondral ossification; fibroblast growth factor receptor signaling pathway; intramembranous ossification; lung development; MAPKKK cascade; peptidyl-tyrosine phosphorylation; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of vascular endothelial growth factor receptor signaling pathway; regulation of endothelial cell proliferation; regulation of phosphoinositide 3-kinase cascade; signal transduction