DAAO active protein

DAAO, 1-347 aa, Human, His tag, E Coli (Bioactivity Validated)

Cat. Num. AAA206324

• Gene Names: DAO; DAAO; OXDA; DAMOX
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: DAAO; 1-347 aa; Human; His tag; E Coli (Bioactivity Validated); D-amino acid oxidase; DAMOX; DAO; OXDA; DAAO active protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL
• Sequence Length: 347

• Applicable Applications for DAAO active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 3.5 units/mg, in which one unit will oxidatively deaminate 1.0 umole of D-alanine to pyruvate per minute at pH 8.5 at 37C, in the presence of catalase.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DAAO active protein

D-amino-acid oxidase (DAAO) is a peroxisomal enzyme which uses flavin adenine dinucleotide (FAD) as a cofactor and oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. It has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging. Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for DAAO active protein

Redox Proteins; Metabolism

References

Kawazoe T., et al. (2006). Protein Sci. 15 (12):2708-17.; Chassande O., et al. (1994). J Biol Chem. 269 (20):14484-9.

NCBI and Uniprot Product Information

• NCBI GI #: 148539837
• NCBI GeneID: 1610
• NCBI Accession #: NP_001908
• NCBI GenBank Nucleotide #: NM_001917.4
• UniProt Accession #: P14920; Q16758; Q8N6R2; B2R7I5
• Molecular Weight: 41.6 kDa (367aa), confirmed by MALDI-TOF
• NCBI Official Full Name: D-amino-acid oxidase
• NCBI Official Synonym Full Names: D-amino acid oxidase
• NCBI Official Symbol: DAO
• NCBI Official Synonym Symbols: DAAO; OXDA; DAMOX
• NCBI Protein Information: D-amino-acid oxidase
• UniProt Protein Name: D-amino-acid oxidase
• UniProt Gene Name: DAO
• UniProt Synonym Gene Names: DAMOX; DAAO; DAMOX; DAO

NCBI Description

This gene encodes the peroxisomal enzyme D-amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. Its biological function is not known; it may act as a detoxifying agent which removes D-amino acids that accumulate during aging. In mice, it degrades D-serine, a co-agonist of the NMDA receptor. This gene may play a role in the pathophysiology of schizophrenia. [provided by RefSeq, Jul 2008]

Uniprot Description

Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.