C-10 Active Protein
C-10, Recombinant, Mouse (MRP-1, Chemokine C-C motif ligand 6, CCL6)
Purity
Highly Purified97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Synonyms
C-10; Recombinant; Mouse (MRP-1; Chemokine C-C motif ligand 6; CCL6); C-10 active protein
Host
E Coli
Purity/Purification
Highly Purified
97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
97% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form/Format
Supplied as a lyophilized powder. No additives.
Biological Activity
Murine C-10 is fully biologically active when compared to standard. The Biological activity is calculated by the chemotaxis assay using Balb/C mouse spleen MNCs. The maximum chemotactic activity was achieved at 100ng/ml.
Dimers and Aggregates
1% as determined by silver-stained SDS-PAGE gel analysis.
Endotoxin Level
0.1ng/ug (IEU/ug) of Murine C-10.
Protein Content
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of Murine C-10 as a Reference Standard.
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of Murine C-10 as a Reference Standard.
Reconstitution
Reconstitute the lyophilized Murine C-10 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for C-10 active protein
Recombinant Murine C-10 is a single, non-glycosylated, polypeptide chain containing 95 amino acids and having a molecular mass of 10710 Dalton.
Murine C-10 (MRP-1, Chemokine (C-C motif ligand 6, CCL6) is a member of the C-C chemokine family of proteins related to macrophage inflammatory protein-1 alpha (MIP-1 alpha). Murine C-10 is a 10.7kD protein containing 95 amino acid residues. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
Murine C-10 (MRP-1, Chemokine (C-C motif ligand 6, CCL6) is a member of the C-C chemokine family of proteins related to macrophage inflammatory protein-1 alpha (MIP-1 alpha). Murine C-10 is a 10.7kD protein containing 95 amino acid residues. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
Product Categories/Family for C-10 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Chain C, Ms2-rna Hairpin (c-10) Complex
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Product Notes
The C-10 (Catalog #AAA650853) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "C-10, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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