Western Blot (WB)
(Western blot analysis of SPINK2 in human testis tissue lysate with SPINK2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Rabbit anti-Human, Mouse SPINK2 Polyclonal Antibody | anti-SPINK2 antibody
SPINK2 Antibody
Gene Names
SPINK2; HUSI-II
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
SPINK2 Antibody is affinity chromatography purified via peptide column.
Synonyms
SPINK2; Polyclonal Antibody; SPINK2 Antibody; HUSI-II; Serine protease inhibitor Kazal-type 2; Acrosin-trypsin inhibitor; serine peptidase inhibitor; Kazal type 2 (acrosin-trypsin inhibitor); anti-SPINK2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SPINK2 antibody is predicted to not cross-react with other SPINK family members.
Purity/Purification
SPINK2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
84
Applicable Applications for anti-SPINK2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
SPINK2 antibody can be used for detection of SPINK2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Optimal dilutions for each application to be determined by the researcher.
Optimal dilutions for each application to be determined by the researcher.
Conjugate
Unconjugated
Immunogen
SPINK2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of mouse SPINK2.
Buffer
SPINK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Homology
Predicted species based on immunogen sequence : Rat (100%)
Preparation and Storage
SPINK2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of SPINK2 in human testis tissue lysate with SPINK2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of SPINK2 in human testis tissue lysate with SPINK2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SPINK2 in mouse testis tissue with SPINK2 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SPINK2 in mouse testis tissue with SPINK2 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of SPINK2 in mouse testis tissue with SPINK2 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of SPINK2 in mouse testis tissue with SPINK2 antibody at 20 μg/mL.)
Related Product Information for anti-SPINK2 antibody
SPINK2 Antibody: Human serine proteinase inhibitor Kazal-type 2 (SPINK2) is required for maintaining normal spermatogenesis and potentially regulates serine protease-mediated apoptosis in male germ cells. It contains a typical kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged in fertility. SPINK2 plays a role in the pathogenesis of hereditary and chronic pancreatitis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 15 kDa
Observed: 18 kDa
Observed: 18 kDa
NCBI Official Full Name
serine protease inhibitor Kazal-type 2 isoform 2
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kazal type 2 (acrosin-trypsin inhibitor)
NCBI Official Symbol
SPINK2
NCBI Official Synonym Symbols
HUSI-II
NCBI Protein Information
serine protease inhibitor Kazal-type 2; epididymis tissue protein Li 172
UniProt Protein Name
Serine protease inhibitor Kazal-type 2
Protein Family
UniProt Gene Name
SPINK2
UniProt Entry Name
ISK2_HUMAN
NCBI Description
This gene encodes a member of the family of serine protease inhibitors of the Kazal type (SPINK). The encoded protein acts as a trypsin and acrosin inhibitor in the genital tract and is localized in the spermatozoa. The protein has been associated with the progression of lymphomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Uniprot Description
SPINK2: Strong inhibitor of acrosin in male and/or female genital tract. Also inhibits trypsin.
Protein type: Secreted, signal peptide; Inhibitor; Secreted
Chromosomal Location of Human Ortholog: 4q12
Cellular Component: extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity
Research Articles on SPINK2
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Product Notes
The SPINK2 spink2 (Catalog #AAA151088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPINK2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPINK2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). SPINK2 antibody can be used for detection of SPINK2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the SPINK2 spink2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPINK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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