AKR1B10 active protein

AKR1B10, human recombinant

Cat. Num. AAA844229

• Gene Names: AKR1B10; HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12
• Applications: SDS-Page
• Purity: >=98%
• Synonyms: AKR1B10; human recombinant; Aldo-keto reductase family 1; member B10; AKR1B11; AKR1B12; ALDRLn; ARL-1; ARL1; HIS; HIS.; AKR1B10 active protein

• Host: E Coli
• Purity/Purification: >=98%
• Form/Format: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol.; Appearance: Liquid
• Concentration: 1 mg/ml (varies by lot)
• Sequence: MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY
• Sequence Length: 316

• Applicable Applications for AKR1B10 active protein: SDS-PAGE
• Biological Activity: The specific activity of AKR1B10 with 5 mM glyceraldehyde is approximately ~100 mU/mg. (Specific activity is calculated by the decrease of absorption at 340 nm in presence of 2-10 ug AKR1B10, 5mM glyceraldehyde, 200 uM NADPH in 0.1 M sodium phosphate buffer, pH 7.5).
• Results: ~100 mU/mg
• Unit Definition: One unit is the amount of enzyme that oxidizes 1.0 umole of NADPH to NADP+ per min. at pH 7.5 at 25 degree C.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -80 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for AKR1B10 active protein

Background: AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications. It has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer.

Product Categories/Family for AKR1B10 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)  

NCBI and Uniprot Product Information

• NCBI GI #: 223468663
• NCBI GeneID: 57016
• NCBI Accession #: NP_064695.3
• NCBI GenBank Nucleotide #: NM_020299.4
• UniProt Accession #: O60218; O75890; Q6FHF3; Q8IWZ1; A4D1P1
• Molecular Weight: 36 kDa
• NCBI Official Full Name: aldo-keto reductase family 1 member B10
• NCBI Official Synonym Full Names: aldo-keto reductase family 1, member B10 (aldose reductase)
• NCBI Official Symbol: AKR1B10
• NCBI Official Synonym Symbols: HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12
• NCBI Protein Information: aldo-keto reductase family 1 member B10
• UniProt Protein Name: Aldo-keto reductase family 1 member B10
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: AKR1B10
• UniProt Synonym Gene Names: AKR1B11; ARP; hARP; SI reductase
• UniProt Entry Name: AK1BA_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

AKR1B10: Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. Belongs to the aldo/keto reductase family.

Protein type: Carbohydrate Metabolism - butanoate; EC 1.1.1.21; Oxidoreductase; Carbohydrate Metabolism - fructose and mannose; EC 1.1.1.-; Lipid Metabolism - linoleic acid

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: lysosome; cytosol

Molecular Function: protein binding; aldo-keto reductase activity; indanol dehydrogenase activity; geranylgeranyl reductase activity; retinal dehydrogenase activity

Biological Process: steroid metabolic process; phototransduction, visible light; digestion; farnesol catabolic process; aldehyde metabolic process; retinoid metabolic process