Rabbit Dual specificity tyrosine-phosphorylation-regulated kinase 1A ELISA Kit | DYRK1A elisa kit

Rabbit Dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A) ELISA Kit

Cat. Num. AAA283002

• Reactivity: Rabbit
• Synonyms: Dual specificity tyrosine-phosphorylation-regulated kinase 1A; Rabbit Dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A) ELISA Kit; DYRK1A elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rabbit DYRK1A. No significant cross-reactivity or interference between Rabbit DYRK1A and analogues was observed.
• Sequence Length: 763

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DYRK1A elisa kit

DYRK1A encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DYRK1A in samples. An antibody specific for DYRK1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDYRK1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DYRK1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DYRK1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 122063444
• UniProt Accession #: P85051
• Molecular Weight: 22,896 Da
• NCBI Official Full Name: Dual specificity tyrosine-phosphorylation-regulated kinase 1A
• UniProt Protein Name: Dual specificity tyrosine-phosphorylation-regulated kinase 1A
• Protein Family: Dual specificity tyrosine-phosphorylation-regulated kinase
• UniProt Gene Name: DYRK1A
• UniProt Synonym Gene Names: MNBH

Uniprot Description

DYRK1A: a dual-specificity protein kinase of the DYRK family. Contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. May play a significant role in a pathway regulating cell proliferation and may be involved in brain development. Its gene is localized in the Down syndrome (DS) critical region of chromosome 21, and is a strong candidate gene for learning defects associated with Down syndrome. Overexpression in mice gives rise to neurological abnormalities similar to those observed in DS. Five splice-variant isoforms have been described.

Protein type: CMGC group; DYRK family; Dyrk1 subfamily; EC 2.7.12.2; Kinase, protein; Protein kinase, CMGC; Protein kinase, dual-specificity (non-receptor)