DYNLL1 recombinant protein

Recombinant Human DYNLL1 Protein

Cat. Num. AAA8248946

• Gene Names: DYNLL1; LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: DYNLL1; Recombinant Human DYNLL1 Protein; DLC1; DNCL1; DNCLC1; HDLC1; Dynein light chain 1; cytoplasmic; 8 kDa dynein light chain; DLC8; Dynein light chain LC8-type 1; Protein inhibitor of neuronal nitric oxide synthase; PIN; DYNLL1 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM Tris-HCl, 200mM NaCl, 1mM DTT, pH 8.0.
• Sequence: MGSSHHHHHH SSGLVPRGSH MCDRKAVIKN ADMSEEMQQD SVECATQALE KYNIEKDIAA HIKKEFDKKY NPTWHCIVGR NFGSYVTHET KHFIYFYLGQ VAILLFKSG
• Sequence Length: 89

• Applicable Applications for DYNLL1 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for DYNLL1 recombinant protein

Recombinant Human DYNLL1 Protein is produced by our E.coli expression system and the target gene encoding Met1-Gly89 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 83267866
• NCBI GeneID: 8655
• NCBI Accession #: NP_001032583.1
• NCBI GenBank Nucleotide #: NM_001037494.1
• UniProt Accession #: P63167; Q15701
• Molecular Weight: 10,366 Da
• NCBI Official Full Name: dynein light chain 1, cytoplasmic
• NCBI Official Synonym Full Names: dynein light chain LC8-type 1
• NCBI Official Symbol: DYNLL1
• NCBI Official Synonym Symbols: LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1
• NCBI Protein Information: dynein light chain 1, cytoplasmic
• UniProt Protein Name: Dynein light chain 1, cytoplasmic
• UniProt Gene Name: DYNLL1
• UniProt Synonym Gene Names: DLC1; DNCL1; DNCLC1; HDLC1; DLC8; PIN
• UniProt Entry Name: DYL1_HUMAN

NCBI Description

Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

LC8: a highly conserved cytoplasmic protein of the dynein light chain family. Cytoplasmic dyneins are large enzyme complexes. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain. Interacts with and inhibits the activity of neuronal nitric oxide synthase (nNOS). A highly conserved protein, showing 92% amino acid similarity with the nematode homolog. Binding of this protein destabilizes the nNOS dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity.

Protein type: Microtubule-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: centrosome; cytoplasm; cytoplasmic dynein complex; cytosol; kinetochore; membrane; nucleus; plasma membrane; signalosome

Molecular Function: protein binding

Biological Process: actin cytoskeleton organization and biogenesis; anatomical structure morphogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class II; ER to Golgi vesicle-mediated transport; female gamete generation; G2/M transition of mitotic cell cycle; macroautophagy; negative regulation of phosphorylation; substantia nigra development