Western Blot (WB)
((1ug/ml) staining of lysate of cell line HEK293 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat WNT3 Polyclonal Antibody | anti-WNT3 antibody
Goat anti-WNT3 Antibody
Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration: 1-3ug/ml.
Immunocytochemsitry/Immunofluorescence This antibody has been successfully used in ICC/IF for mouse hippocampal primary astrocytes as described in the following paper: Okamoto et al, FASEB J.2011 Jul 11; PMID: 21746862.
Western Blot (WB)
((1ug/ml) staining of lysate of cell line HEK293 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of lysate of cell line HEK293 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq, Jul 2008]
Uniprot Description
WNT3: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA). Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects. Belongs to the Wnt family.
Protein type: Secreted; Oncoprotein; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix
Molecular Function: frizzled binding; protein binding; protein domain specific binding; receptor agonist activity
Biological Process: anterior/posterior axis specification; axon guidance; cell fate commitment; cell morphogenesis; dorsal/ventral axis specification; embryonic forelimb morphogenesis; embryonic hindlimb morphogenesis; gamete generation; limb bud formation; mesoderm formation; negative regulation of axon extension involved in axon guidance; neuron differentiation; positive regulation of collateral sprouting in the absence of injury; positive regulation of Wnt receptor signaling pathway; Spemann organizer formation at the anterior end of the primitive streak; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin
Disease: Tetraamelia, Autosomal Recessive
Research Articles on WNT3
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Product Notes
The WNT3 wnt3 (Catalog #AAA421042) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-WNT3 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's WNT3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF). Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. Recommended concentration, 2-4ug/ml. Western Blot: Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration: 1-3ug/ml. Immunocytochemsitry/Immunofluorescence This antibody has been successfully used in ICC/IF for mouse hippocampal primary astrocytes as described in the following paper: Okamoto et al, FASEB J.2011 Jul 11; PMID: 21746862. Researchers should empirically determine the suitability of the WNT3 wnt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: CGRGHNTRTE KRKEK. It is sometimes possible for the material contained within the vial of "WNT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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