Human Dual specificity protein phosphatase 3 ELISA Kit | DUSP3 elisa kit

Human Dual specificity protein phosphatase 3 ELISA Kit

Cat. Num. AAA2887773

• Gene Names: DUSP3; VHR
• Reactivity: Human
• Synonyms: Dual specificity protein phosphatase 3; Human Dual specificity protein phosphatase 3 ELISA Kit; Dual specificity protein phosphatase VHR; Vaccinia H1-related phosphatase; VHR; DUSP3; 3.1.3.16; DUSP3 elisa kit

• Reactivity: Human
• Sequence Length: 185

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/mL
• Sensitivity: 0.058ng/ml
• Intra-Assay CV: <=5.5%
• Inter-Assay CV: <=8.2%
• Recovery: 90%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 4758208
• NCBI GeneID: 1845
• NCBI Accession #: NP_004081.1
• NCBI GenBank Nucleotide #: NM_004090.3
• Molecular Weight: 16,163 Da
• NCBI Official Full Name: dual specificity protein phosphatase 3
• NCBI Official Synonym Full Names: dual specificity phosphatase 3
• NCBI Official Symbol: DUSP3
• NCBI Official Synonym Symbols: VHR
• NCBI Protein Information: dual specificity protein phosphatase 3
• UniProt Protein Name: Dual specificity protein phosphatase 3
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP3
• UniProt Synonym Gene Names: VHR; VHR
• UniProt Entry Name: DUS3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008]

Uniprot Description

DUSP3: a non-receptor, dual-specificity phosphoprotein phosphatase (DUSP). Different members of the DUSP family show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP3 inactivates the mitogen-activated kinases Erk2 and Jnk and is phosphorylated by ZAP-70. Phosphorylation by ZAP-70 is required for DUSP3 to inhibit the Erk2-Elk-1 pathway.

Protein type: EC 3.1.3.16; EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; cytosol; immunological synapse; nucleoplasm; nucleus

Molecular Function: MAP kinase phosphatase activity; phosphoric monoester hydrolase activity; protein kinase binding; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity

Biological Process: dephosphorylation; inactivation of MAPK activity; negative regulation of JNK cascade; negative regulation of MAPKKK cascade; negative regulation of T cell activation; negative regulation of T cell receptor signaling pathway; positive regulation of mitotic cell cycle