SDS-Page
DSTN recombinant protein
DSTN, 1-165aa, Human, His tag, E Coli
Gene Names
DSTN; ADF; ACTDP; HEL32; bA462D18.2
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
DSTN; 1-165aa; Human; His tag; E Coli; Destrin (actin depolymerizing factor); ACTDP; ADF; bA462D18.2; DSTN recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MASGVQVADE VCRIFYDMKV RKCSTPEEIK KRKKAVIFCL SADKKCIIVE EGKEILVGDV GVTITDPFKH FVGMLPEKDC RYALYDASFE TKESRKEELM FFLWAPELAP LKSKMIYASS KDAIKKKFQG IKHECQANGP EDLNRACIAE KLGGSLIVAF EGCPVLEHHH HHH
Sequence Length
165
Applicable Applications for DSTN recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for DSTN recombinant protein
Destrin (actin depolymerizing factor), also known as DSTN, is a member of the ADF/Cofilin/destrin superfamily that has the ability to rapidly depolymerize F-Actin in a stoichiometric manner. DSTN is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filaments in vitro. It is found in various epithelial and endothelial cells but is practically absent from adult mouse heart and skeletal muscle cells. DSTN shares 71% sequence homology with Cofilin, however the two proteins differ in their interaction with Actin. Recombinant human DSTN protein, fused to His-tag at C-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for DSTN recombinant protein
References
Nishida E., et al. (1985) Biochemistry. 24:6624-6630.; Kanamori T., et al. (1995) J Biol Chem. 270: 8061-8067.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19.5 kDa (173aa) confirmed by MALDI-TOF
NCBI Official Full Name
destrin isoform a
NCBI Official Synonym Full Names
destrin, actin depolymerizing factor
NCBI Official Symbol
DSTN
NCBI Official Synonym Symbols
ADF; ACTDP; HEL32; bA462D18.2
NCBI Protein Information
destrin
UniProt Protein Name
Destrin
Protein Family
UniProt Gene Name
DSTN
UniProt Synonym Gene Names
ACTDP; DSN; ADF
NCBI Description
The product of this gene belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.
Research Articles on DSTN
Similar Products
Product Notes
The DSTN dstn (Catalog #AAA204618) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's DSTN can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the DSTN dstn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MASGVQVADE VCRIFYDMKV RKCSTPEEIK KRKKAVIFCL SADKKCIIVE EGKEILVGDV GVTITDPFKH FVGMLPEKDC RYALYDASFE TKESRKEELM FFLWAPELAP LKSKMIYASS KDAIKKKFQG IKHECQANGP EDLNRACIAE KLGGSLIVAF EGCPVLEHHH HHH. It is sometimes possible for the material contained within the vial of "DSTN, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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