Western Blot (WB)
(Western blotting of DR6 / Fc recombinant protein. Reducing conditions. Lane 1 to 4: immunostaining with rabbit polyclonal antibody specific to DR6 (EC). Detection with Goat anti-rabbit / HRP secondary antibody. Amount of DR6 / Fc recombinant protein loaded on each lane: (1) 150 ng, (2) 75 ng, (3) 30 ng, (4) 15 ng per lane.)
DR6/Fc recombinant protein
Recombinant human DR6/Fc Chimera
Applications
Western Blot
Purity
The protein was purified from cell culture supernatant by protein-A affinity chromatography and lyophilized.
Synonyms
DR6/Fc; Recombinant human DR6/Fc Chimera; DR6/Fc recombinant protein
Host
HEK 293 cells
Purity/Purification
The protein was purified from cell culture supernatant by protein-A affinity chromatography and lyophilized.
Form/Format
Purified. Lyophilized from a 0.22um filtered solution in phosphate buffered saline (PBS).
Applicable Applications for DR6/Fc recombinant protein
Western Blot (WB)
Preparation and Storage
Store lyophilized product in freezer (-20 degree C to -80 degree C). The expiration date of unopened product vial is indicated on the vial label. After reconstitution prepare suitable aliquots. Reconstituted product is stable for at least 6 months when stored in freezer (-20 degree C to -80 degree C). Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blotting of DR6 / Fc recombinant protein. Reducing conditions. Lane 1 to 4: immunostaining with rabbit polyclonal antibody specific to DR6 (EC). Detection with Goat anti-rabbit / HRP secondary antibody. Amount of DR6 / Fc recombinant protein loaded on each lane: (1) 150 ng, (2) 75 ng, (3) 30 ng, (4) 15 ng per lane.)
Western Blot (WB)
(Western blotting of DR6 / Fc recombinant protein. Reducing conditions. Lane 1 to 4: immunostaining with rabbit polyclonal antibody specific to DR6 (EC). Detection with Goat anti-rabbit / HRP secondary antibody. Amount of DR6 / Fc recombinant protein loaded on each lane: (1) 150 ng, (2) 75 ng, (3) 30 ng, (4) 15 ng per lane.)
SDS-Page
(SDS-PAGE Electrophoresis of DR6 / Fc recombinant protein (12% separating gel, 6% stacking gel; reducing conditions). Lane 1 to 5: DR6 / Fc recombinant protein (2000 ng, 1000 ng, 500 ng, 200 ng, 100 ng per lane). Staining with Coomassie Blue.)
SDS-Page
(SDS-PAGE Electrophoresis of DR6 / Fc recombinant protein (12% separating gel, 6% stacking gel; reducing conditions). Lane 1 to 5: DR6 / Fc recombinant protein (2000 ng, 1000 ng, 500 ng, 200 ng, 100 ng per lane). Staining with Coomassie Blue.)
Related Product Information for DR6/Fc recombinant protein
Recombinant human DR6 (TNFRSF21)/Fc Chimera is produced in HEK 293 cells. The extracellular domain of human DR6 (aa 1 - 212) is fused to the Fc portion of human IgG1.
A calculated molecular mass of fussion protein is 40kDa. As a result of glycosylation, the recombinant protein migrates as a 50kDa protein in SDS-PAGE under reducing conditions.
A calculated molecular mass of fussion protein is 40kDa. As a result of glycosylation, the recombinant protein migrates as a 50kDa protein in SDS-PAGE under reducing conditions.
References
*Pan G et al., Identification and Functional Application (FUNC)tional characterization of DR6, a novel death domain-containing TNF receptor. FEBS Lett. 1998 431(3):351-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/9714541dopt=Abstract
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Product Notes
The DR6/Fc (Catalog #AAA1802110) is a Recombinant Protein produced from HEK 293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DR6/Fc can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DR6/Fc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DR6/Fc, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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