DPYSL5 recombinant protein

Purified Recombinant DPYSL5 protein (His tag)

Cat. Num. AAA5308619

• Gene Names: DPYSL5; CRAM; CRMP5; Ulip6; CRMP-5
• Applications: SDS-Page, Western Blot
• Purity: > 85% pure; Purified using proprietary chromatographic techniques
• Synonyms: DPYSL5; Purified Recombinant DPYSL5 protein (His tag); BMP binding endothelial regulator protein; BMP-binding endothelial regulator precursor protein protein; BMPER protein; bone morphogenetic protein-binding endothelial cell precursor-derived regulator protein; CRIM3 protein; crossveinless 2 protein; crossveinless-2 protein; CV-2 protein; CV2 protein; Q8N8U9 protein; DPYSL5 recombinant protein

• Host: Human
• Purity/Purification: > 85% pure; Purified using proprietary chromatographic techniques
• Form/Format: Frozen liquid in 50mM Tris-HCI, pH 8.5, with 150mM NaCl, 2mM DTT, 1mM EDTA, and 10% Glycerol.
• Sequence Length: 1-564 aa

• Applicable Applications for DPYSL5 recombinant protein: SDS-PAGE, Western Blot (WB)
• Application Notes: Currently being validated in ELISA
• Grade: Recombinant
• Biohazard: Use standard laboratory precautions when handling this material
• Species: Human
• Residues: 1-564 aa
• Biological Significance: DPYSL5 is a secreted protein that interacts with, and inhibits bone morphogenetic protein (BMP) function. It has been shown to inhibit BMP2- and BMP4-dependent osteoblast differentiation and BMP-dependent differentiation of the chondrogenic cells. DPYSL5 is a member of the collapsin response mediator protein family (CRMPs) which are involved in an autoimmune disease associated with small cell lung carcinoma. Members of the CRMP family, such as DPYSL5, are believed to play a role in growth cone guidance during neural development.
• Expression: DPYSL5 is highly expressed in lung, and brain and also in primary chondrocytes.
• Implications in Disease: Defects in DPYSL5 are the cause of diaphanospondylodysostosis (DSD), a rare, recessively inherited, perinatal lethal skeletal disorder, the characteristics of which include a small chest, abnormal vertebral segmentation, as well as craniofacial abnormalities.
• Sequence Information: DPYSL5 is composed of 564 amino acids with a molecular weight of 65 kDa. CV2 contains 1 TIL (trypsin inhibitory-like) domain, 5 VWFC domains and 1 VWFD domain.
• Post-Translational Modifications: Glycosylation of DPYSL5 occurs at Asn116, Asn247, Asn255, Asn318, Asn441
• Preparation and Storage: Ships on dry ice. Upon receipt store at -70°C

Western Blot (WB)

Western Blot (WB)

Product Categories/Family for DPYSL5 recombinant protein

Proteases; Inhibitors; Enzymes; Conjugated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 12804043
• NCBI GeneID: 56896
• Molecular Weight: 65 kDa
• NCBI Official Full Name: DPYSL5 protein
• NCBI Official Synonym Full Names: dihydropyrimidinase like 5
• NCBI Official Symbol: DPYSL5
• NCBI Official Synonym Symbols: CRAM; CRMP5; Ulip6; CRMP-5
• NCBI Protein Information: dihydropyrimidinase-related protein 5
• UniProt Protein Name: Dihydropyrimidinase-related protein 5
• Protein Family: Dihydropyrimidinase-related protein
• UniProt Gene Name: DPYSL5
• UniProt Synonym Gene Names: CRMP5; ULIP6; DRP-5; CRAM; CRMP-5; ULIP-6

NCBI Description

This gene encodes a member of the CRMP (collapsing response mediator protein) family thought to be involved in neural development. Antibodies to the encoded protein were found in some patients with neurologic symptoms who had paraneoplastic syndrome. A pseudogene of this gene is found on chromosome 11. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]

Uniprot Description

CRMP-5: May have a function in neuronal differentiation and/or axon growth. Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: cytosol

Molecular Function: protein binding

Biological Process: nervous system development; signal transduction