Mouse dynamin 1-like ELISA Kit | DNM1L elisa kit

Mouse Dynamin-1-like protein, DNM1L ELISA Kit

Cat. Num. AAA9323164

• Gene Names: Dnm1l; Dlp1; Drp1; Dnmlp1; python; AI450666; 6330417M19Rik
• Reactivity: Mouse
• Synonyms: dynamin 1-like; Mouse Dynamin-1-like protein; DNM1L ELISA Kit; Mouse Dynamin-1-like protein (DNM1L) ELISA kit; DLP1; DRP1; DVLP; DYMPLE; DYNIV-11; FLJ41912; HDYNIV; VPS1; Dnm1p/Vps1p-like protein; dynamin family member proline-rich carboxyl-terminal domain less; dynamin-like protein 4; dynamin-like protein IV; d; DNM1L elisa kit

• Reactivity: Mouse

• Assay Type: Quantitative Sandwich
• Detection Range: 0.5ng/ml-16ng/ml
• Sensitivity: 0.1ng/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for DNM1L elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used for determine the level of DNM1L (hereafter termed "analyte") in undiluted original Mouse serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 71061455
• NCBI GeneID: 74006
• NCBI Accession #: NP_001021118.1
• NCBI GenBank Nucleotide #: NM_001025947.2
• UniProt Accession #: Q8K1M6; Q8BNQ5; Q8BQ64; Q8CGD0; Q8K1A1
• Molecular Weight: 82,658 Da
• NCBI Official Full Name: dynamin-1-like protein isoform b
• NCBI Official Synonym Full Names: dynamin 1-like
• NCBI Official Symbol: Dnm1l
• NCBI Official Synonym Symbols: Dlp1; Drp1; Dnmlp1; python; AI450666; 6330417M19Rik
• NCBI Protein Information: dynamin-1-like protein; dymple; dynamin-related protein 1; dynamin family member proline-rich carboxyl-terminal domain less
• UniProt Protein Name: Dynamin-1-like protein
• Protein Family: Dynamin-1-like protein
• UniProt Gene Name: Dnm1l
• UniProt Synonym Gene Names: Drp1; Dymple
• UniProt Entry Name: DNM1L_MOUSE

NCBI Description

This gene encodes a member of the dynamin family. The encoded protein is localized to the cytoplasm and mitochondrial membrane, is involved in mitochondrial and peroxisomal division, and is essential for mitochondrial fission. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 2. [provided by RefSeq, Feb 2013]

Uniprot Description

DRP1: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis- dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Can self-assemble in multimeric ring-like structures. Interacts with BCL2L1; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with FIS1. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in ite B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria. Interacts witn MID49 and MID51. Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. Belongs to the dynamin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Endoplasmic reticulum; Mitochondrial; Motor; Microtubule-binding; Hydrolase; EC 3.6.5.5

Cellular Component: Golgi apparatus; microtubule; protein complex; intracellular membrane-bound organelle; mitochondrion; coated pit; cytosol; mitochondrial outer membrane; membrane; perinuclear region of cytoplasm; cytoplasm; peroxisome; synapse; cytoplasmic vesicle; cell junction

Molecular Function: GTPase activity; identical protein binding; protein binding; protein homodimerization activity; GTP binding; hydrolase activity; ubiquitin protein ligase binding; nucleotide binding; lipid binding

Biological Process: peroxisome fission; mitochondrial fission; release of cytochrome c from mitochondria; positive regulation of protein secretion; metabolic process; positive regulation of apoptosis; programmed cell death; regulation of protein oligomerization; endocytosis; mitochondrial fragmentation during apoptosis; protein homotetramerization