Rat Deoxyribonuclease-1 ELISA Kit | Dnase1 elisa kit

Rat Deoxyribonuclease-1 ELISA Kit

Cat. Num. AAA762169

• Gene Names: DNASE1; DNL1; DRNI
• Reactivity: Rat
• Synonyms: Deoxyribonuclease-1; Rat Deoxyribonuclease-1 ELISA Kit; Dnase1/DNase-I; Dnase1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dnase1. No significant cross-reactivity or interference between Dnase1 and analogues was observed.
• Sequence Length: 282

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.391-25ng/ml
• Sensitivity: 0.234ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Dnase1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 21361254
• NCBI GeneID: 1773
• NCBI Accession #: NP_005214.2
• NCBI GenBank Nucleotide #: NM_005223.3
• Molecular Weight: 19,771 Da
• NCBI Official Full Name: deoxyribonuclease-1
• NCBI Official Synonym Full Names: deoxyribonuclease I
• NCBI Official Symbol: DNASE1
• NCBI Official Synonym Symbols: DNL1; DRNI
• NCBI Protein Information: deoxyribonuclease-1
• UniProt Protein Name: Deoxyribonuclease-1
• Protein Family: Deoxyribonuclease
• UniProt Gene Name: DNASE1
• UniProt Synonym Gene Names: DNL1; DRNI; DNase I
• UniProt Entry Name: DNAS1_HUMAN

NCBI Description

This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family.

Protein type: Secreted; Motility/polarity/chemotaxis; EC 3.1.21.1; Secreted, signal peptide; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular region; nuclear envelope; nucleus

Molecular Function: actin binding; deoxyribonuclease I activity; protein binding

Biological Process: apoptosis; DNA catabolic process

Disease: Systemic Lupus Erythematosus