Chicken Dentin Matrix Protein 1 ELISA Kit | DMP1 elisa kit

Chicken Dentin Matrix Protein 1 ELISA Kit

Cat. Num. AAA755802

• Gene Names: DMP1; ARHP; ARHR; DMP-1
• Reactivity: Chicken
• Synonyms: Dentin Matrix Protein 1; Chicken Dentin Matrix Protein 1 ELISA Kit; DMP1 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DMP1 elisa kit

Principle of the assay: DMP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DMP1 antibody and an DMP1-HRP conjugate. The assay sample and buffer are incubated together with DMP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DMP1 concentration since DMP1 from samples and DMP1-HRP conjugate compete for the anti-DMP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DMP1 from the sample, fewer sites are left to bind DMP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DMP1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4758172
• NCBI GeneID: 1758
• NCBI Accession #: NP_004398.1
• NCBI GenBank Nucleotide #: NM_004407.3
• Molecular Weight: 54,115 Da
• NCBI Official Full Name: dentin matrix acidic phosphoprotein 1 isoform 1
• NCBI Official Synonym Full Names: dentin matrix acidic phosphoprotein 1
• NCBI Official Symbol: DMP1
• NCBI Official Synonym Symbols: ARHP; ARHR; DMP-1
• NCBI Protein Information: dentin matrix acidic phosphoprotein 1; dentin matrix protein 1
• UniProt Protein Name: Dentin matrix acidic phosphoprotein 1
• Protein Family: Dentin matrix acidic phosphoprotein
• UniProt Gene Name: DMP1
• UniProt Synonym Gene Names: DMP-1; Dentin matrix protein 1
• UniProt Entry Name: DMP1_HUMAN

NCBI Description

Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DMP1: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. Defects in DMP1 are the cause of rickets hypophosphatemic autosomal recessive type 1 (ARHR1). A hereditary form of hypophosphatemic rickets, a disorder of proximal renal tubule function that causes phosphate loss, hypophosphatemia and skeletal deformities, including rickets and osteomalacia unresponsive to vitamin D. Symptoms are bone pain, fractures and growth abnormalities. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: nucleoplasm; proteinaceous extracellular matrix; intracellular membrane-bound organelle; cytoplasm; extracellular region; nucleolus; nucleus

Molecular Function: integrin binding; extracellular matrix binding; calcium ion binding

Biological Process: extracellular matrix organization and biogenesis; ossification; biomineral formation

Disease: Hypophosphatemic Rickets, Autosomal Recessive, 1