DKKL1 recombinant protein

DKKL1 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120514

• Gene Names: DKKL1; SGY; CT34; SGY1; SGY-1
• Purity: >70% as determined by SDS-PAGE
• Synonyms: DKKL1; DKKL1 Protein; Human; Recombinant (hFc Tag); Human DKKL1/Soggy-1/SGY-1 Protein (Fc Tag); CT34 Protein; SGY Protein; SGY-1 Protein; SGY1 Protein; dickkopf-like 1; DKKL1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >70% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala31-Leu242

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu
• Tag: N-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the secreted form of human DKKL1 (Q9UK85)(Ala 31-Leu 242) was expressed with the fused Fc region of human IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for DKKL1 recombinant protein

Background: Dickkopf-like 1 (DKKL1) or soggy 1, is a glycoprotein unique to mammals that is expressed primarily in developing spermatocytes and localized in the acrosome of mature sperm. It is also expressed in the trophectoderm/placental lineage. This glycoprotein is secreted by postmeiotic male germ cells. DKKL1 is a member of the Dickkopf (DKK) family, a group of proteins that are characterized as secreted antagonists of Wnt signal transduction proteins. In mammals, embryos lacking DKKL1 protein developed into viable, fertile adults. DKKL1, either directly or indirectly, facilitates the ability of sperm to penetrate the zona pellucid. DKKL1 is related to the sperm apoptotic procession. Molecular analyses identified the Fas death ligand (FasL) as a target for DkkL1 pro-apoptotic activity in adult mice. DKKL1 is considered as a negative regulator of adult testic homeostasis and identifies a novel, DKKL1/FasL- dependent, regulation that specifically controls the number of postpubertal spermatocytes.

References

Niehrs C. (2006) Function and biological roles of the Dickkopf family of Wnt modulators. Oncogene. 25 (57): 7469-81.Kohn MJ, et al. (2010) The Acrosomal Protein Dickkopf-Like 1 (DKKL1) Facilitates Sperm Penetration Of The Zona Pellucida. Fertil Steril. 93 (5): 1533-7.KOHN MJ, et al. (2005) DKKL1 (Soggy), A Dickkopf Family Member, Localizes to the Acrosome During Mammalian Spermatogenesis. Mol Reprod Dev. 71 (4): 516-22.

NCBI and Uniprot Product Information

• NCBI GI #: 308818218
• NCBI GeneID: 27120
• NCBI Accession #: NP_001184230.1
• NCBI GenBank Nucleotide #: NM_001197301.1
• UniProt Accession #: Q9UK85
• Molecular Weight: 27,007 Da
• NCBI Official Full Name: dickkopf-like protein 1 isoform 2
• NCBI Official Synonym Full Names: dickkopf like acrosomal protein 1
• NCBI Official Symbol: DKKL1
• NCBI Official Synonym Symbols: SGY; CT34; SGY1; SGY-1
• NCBI Protein Information: dickkopf-like protein 1
• UniProt Protein Name: Dickkopf-like protein 1
• Protein Family: Dickkopf-like protein
• UniProt Gene Name: DKKL1
• UniProt Synonym Gene Names: CT34; SGY-1

NCBI Description

The dickkopf protein family interacts with the Wnt signaling pathway and its members are characterized by two conserved cysteine-rich domains. This gene encodes a secreted protein that has low sequence similarity to the dickkopf-3 protein. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

Involved in fertilization by facilitating sperm penetration of the zona pellucida. May promotes spermatocyte apoptosis, thereby limiting sperm production. In adults, may reduces testosterone synthesis in Leydig cells. Is not essential either for development or fertility.