Human Dickkopf-like protein 1 ELISA Kit | DKKL1 elisa kit

Human Dickkopf-like protein 1 ELISA Kit

Cat. Num. AAA762103

• Gene Names: DKKL1; SGY; CT34; SGY1; SGY-1
• Reactivity: Human
• Synonyms: Dickkopf-like protein 1; Human Dickkopf-like protein 1 ELISA Kit; DKKL1/Cancer/testis antigen 34/CT34/Protein soggy-1/SGY-1/DKKL1/SGY1/UNQ735/PRO1429; DKKL1 elisa kit

• Reactivity: Human
• Sequence Length: 242

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for DKKL1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human DKKL1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human DKKL1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human DKKL1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman DKKL1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 7657554
• NCBI GeneID: 27120
• NCBI Accession #: NP_055234.1
• NCBI GenBank Nucleotide #: NM_014419.3
• Molecular Weight: 27,007 Da
• NCBI Official Full Name: dickkopf-like protein 1 isoform 1
• NCBI Official Synonym Full Names: dickkopf like acrosomal protein 1
• NCBI Official Symbol: DKKL1
• NCBI Official Synonym Symbols: SGY; CT34; SGY1; SGY-1
• NCBI Protein Information: dickkopf-like protein 1
• UniProt Protein Name: Dickkopf-like protein 1
• Protein Family: Dickkopf-like protein
• UniProt Gene Name: DKKL1
• UniProt Synonym Gene Names: SGY1; CT34; SGY-1
• UniProt Entry Name: DKKL1_HUMAN

NCBI Description

The dickkopf protein family interacts with the Wnt signaling pathway and its members are characterized by two conserved cysteine-rich domains. This gene encodes a secreted protein that has low sequence similarity to the dickkopf-3 protein. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

DKKL1:

Protein type: Secreted; Cancer Testis Antigen (CTA); Secreted, signal peptide; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular space

Molecular Function: signal transducer activity

Biological Process: anatomical structure morphogenesis; positive regulation of defense response to virus by host; positive regulation of fat cell differentiation; signal transduction