Antigen-Down Assay Diluent
Antigen-Down Assay Diluent
Synonyms
Antigen-Down Assay Diluent; Antigen-Down Assay Diluent diluent
Form/Format
Supplied at 1X.
Application Notes
1. Once the capture antigen has been coated onto the plate and the plate has been properly blocked with one of ICT's stabilizing blocker products, pipette 50-100 uL of Antigen-Down Assay Diluent into each of the wells of the ELISA plate using a multi-channel pipettor. The Assay Diluent is always added prior to adding the test and control serum/plasma samples to plate wells.
2. The control and test serum/plasma samples should be diluted to the proper well concentrations using one of ICT's three different Sample Diluent products. Using an ICT Sample Diluent to dilute the serum and plasma samples will also serve to help minimize the degree of nonspecific IgG binding to the antigen coated and blocked ELISA plate wells. The test (sera and plasma) samples, diluted typically to 1:40 or greater in a sample diluent, should be incubated in the antigen coated ELISA plates for 30-60 minutes at room temperature or 37 degree C.
2. The control and test serum/plasma samples should be diluted to the proper well concentrations using one of ICT's three different Sample Diluent products. Using an ICT Sample Diluent to dilute the serum and plasma samples will also serve to help minimize the degree of nonspecific IgG binding to the antigen coated and blocked ELISA plate wells. The test (sera and plasma) samples, diluted typically to 1:40 or greater in a sample diluent, should be incubated in the antigen coated ELISA plates for 30-60 minutes at room temperature or 37 degree C.
pH
7.4 at 1X
Contains
Mammalian Proteins.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for Antigen-Down Assay Diluent diluent
Description: Antigen-Down Assay Diluent is formulated for use with serum and plasma samples tested in antigen-down ELISA formats. Proprietary additives have been included to reduce the level of nonspecific IgG adsorption to antigen-coated and blocked ELISA well surfaces, as well as enhancing the specific anti-analyte/antigen-antibody signal without denaturing the plate adsorbed antigen molecules.
Background: Assay Diluents are proprietary ELISA development additives designed to optimize antigen-down ELISA performance by equalizing any differences between the sample matrices (serum, plasma, urine, cell culture fluid) and the calibrator diluent used to generate the standard curve of the ELISA. Assay Diluents are intended to be added to each well of the plate (~100uL/well) just before the addition of the test samples.
Antigen-Down Assay Diluent is formulated for use with serum and plasma samples tested in antigen-down ELISA formats. Proprietary additives have been included in Antigen-Down Assay Diluent to reduce the level of nonspecific IgG adsorption to antigen-coated and blocked ELISA well surfaces, as well as enhancing the specific anti-analyte/antigen antibody signal without denaturing the plate adsorbed antigen molecules.
A mammalian protein-based buffer and a proprietary detergent additive are incorporated in the antigen-down formulation to minimize the nonspecific background signal that often occurs when analyzing human, porcine, or bovine serum samples. Antigen-Down Assay Diluent also contains the highest molar concentration of calcium chelating agents of all assay diluent product formulations.
Formulated to inhibit interference from complement and thrombin present in serum and plasma samples, Antigen-Down Assay Diluent can also be used as a Sample Diluent for plasma sample analysis in sandwich ELISA configurations. An antimicrobial agent is included in the formulation to allow for bench-top use and storage stability.
Background: Assay Diluents are proprietary ELISA development additives designed to optimize antigen-down ELISA performance by equalizing any differences between the sample matrices (serum, plasma, urine, cell culture fluid) and the calibrator diluent used to generate the standard curve of the ELISA. Assay Diluents are intended to be added to each well of the plate (~100uL/well) just before the addition of the test samples.
Antigen-Down Assay Diluent is formulated for use with serum and plasma samples tested in antigen-down ELISA formats. Proprietary additives have been included in Antigen-Down Assay Diluent to reduce the level of nonspecific IgG adsorption to antigen-coated and blocked ELISA well surfaces, as well as enhancing the specific anti-analyte/antigen antibody signal without denaturing the plate adsorbed antigen molecules.
A mammalian protein-based buffer and a proprietary detergent additive are incorporated in the antigen-down formulation to minimize the nonspecific background signal that often occurs when analyzing human, porcine, or bovine serum samples. Antigen-Down Assay Diluent also contains the highest molar concentration of calcium chelating agents of all assay diluent product formulations.
Formulated to inhibit interference from complement and thrombin present in serum and plasma samples, Antigen-Down Assay Diluent can also be used as a Sample Diluent for plasma sample analysis in sandwich ELISA configurations. An antimicrobial agent is included in the formulation to allow for bench-top use and storage stability.
Product Categories/Family for Antigen-Down Assay Diluent diluent
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Product Notes
The Antigen-Down Assay Diluent (Catalog #AAA258393) is a Diluent and is intended for research purposes only. The product is available for immediate purchase. 1. Once the capture antigen has been coated onto the plate and the plate has been properly blocked with one of ICT's stabilizing blocker products, pipette 50-100 uL of Antigen-Down Assay Diluent into each of the wells of the ELISA plate using a multi-channel pipettor. The Assay Diluent is always added prior to adding the test and control serum/plasma samples to plate wells. 2. The control and test serum/plasma samples should be diluted to the proper well concentrations using one of ICT's three different Sample Diluent products. Using an ICT Sample Diluent to dilute the serum and plasma samples will also serve to help minimize the degree of nonspecific IgG binding to the antigen coated and blocked ELISA plate wells. The test (sera and plasma) samples, diluted typically to 1:40 or greater in a sample diluent, should be incubated in the antigen coated ELISA plates for 30-60 minutes at room temperature or 37 degree C. Researchers should empirically determine the suitability of the Antigen-Down Assay Diluent for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Antigen-Down Assay Diluent, Diluent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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