DHH recombinant protein

DHH, 23-198aa, Human, His tag, E Coli

Cat. Num. AAA203272

• Gene Names: DHH; GDXYM; HHG-3; SRXY7
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: DHH; 23-198aa; Human; His tag; E Coli; desert hedgehog preproprotein; Drosophila; HHG-3; MGC35145; Desert hedgehog protein N-product; desert hedgehog preproprotein Desert hedgehog; Desert hedgehog (Drosophila) homolog; Desert hedgehog homolog (Drosophila); Desert hedgehog protein precursor.; DHH recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in 20mM MES pH 5.5, 0.5mM DTT, 20% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MCGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG
• Sequence Length: 396

• Applicable Applications for DHH recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 2-8 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C to -80 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DHH recombinant protein

DHH belongs to the hedgehog family. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N- terminal domain is the active species in both local and long-range signaling, whereas the C-terminal domain has no signaling activity. This protein is produced by Sertoli cells and may be involved in both male gonadal differentiation and perineurial development. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. Recombinant DHH protein was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for DHH recombinant protein

Signal Transduction

References

Chen YJ, et al. (2007). Cell Cycle. 6 (15):1826-30; Van den Brink GR., et al. (2007). Physiol Rev. 87 (4):1343-75;

NCBI and Uniprot Product Information

• NCBI GI #: 19482158
• NCBI GeneID: 50846
• NCBI Accession #: NP_066382
• NCBI GenBank Nucleotide #: NM_021044.3
• UniProt Accession #: O43323; Q15794
• Molecular Weight: 22kDa (197aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: desert hedgehog protein preproprotein
• NCBI Official Synonym Full Names: desert hedgehog
• NCBI Official Symbol: DHH
• NCBI Official Synonym Symbols: GDXYM; HHG-3; SRXY7
• NCBI Protein Information: desert hedgehog protein
• UniProt Protein Name: Desert hedgehog protein
• Protein Family: Desert hedgehog protein
• UniProt Gene Name: DHH
• UniProt Synonym Gene Names: DHH

NCBI Description

This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq, May 2010]

Uniprot Description

Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development.