Human 24-dehydrocholesterol reductase ELISA Kit | DHCR24 elisa kit

Human Delta (24) -sterol reductase, DHCR24 ELISA Kit

Cat. Num. AAA9328939

• Gene Names: DHCR24; DCE; SELADIN1; Nbla03646; seladin-1
• Reactivity: Human
• Synonyms: 24-dehydrocholesterol reductase; Human Delta (24) -sterol reductase; DHCR24 ELISA Kit; Human Delta (24) -sterol reductase (DHCR24) ELISA kit; KIAA0018; Nbla03646; SELADIN1; seladin-1; 3 beta-hydroxysterol delta 24-reductase; diminuto/dwarf1 homolog; putative protein product of Nbla03646; selective AD indicator 1; DHCR24 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between this analyte and analogues is observed.

• Samples: Undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
• Assay Type: Sandwich
• Detection Range: 0.25 ng/ml - 8 ng/ml.
• Sensitivity: 0.1ng/ml.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for DHCR24 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of DHCR24 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 13375618
• NCBI GeneID: 1718
• NCBI Accession #: NP_055577.1
• NCBI GenBank Nucleotide #: NM_014762.3
• UniProt Accession #: Q15392; Q9HBA8; D3DQ51
• Molecular Weight: 60,101 Da
• NCBI Official Full Name: delta(24)-sterol reductase
• NCBI Official Synonym Full Names: 24-dehydrocholesterol reductase
• NCBI Official Symbol: DHCR24
• NCBI Official Synonym Symbols: DCE; SELADIN1; Nbla03646; seladin-1
• NCBI Protein Information: delta(24)-sterol reductase; delta(24)-sterol reductase; seladin 1; diminuto/dwarf1 homolog; selective AD indicator 1; desmosterol-to-cholesterol enzyme; 3 beta-hydroxysterol delta 24-reductase; 3-beta-hydroxysterol delta-24-reductase
• UniProt Protein Name: Delta(24)-sterol reductase
• Protein Family: Delta(24)-sterol reductase
• UniProt Gene Name: DHCR24
• UniProt Synonym Gene Names: KIAA0018
• UniProt Entry Name: DHC24_HUMAN

NCBI Description

This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008]

Uniprot Description

Diminuto: the enzyme 3-beta-hydroxysterol delta-24-reductase (DHCR24), a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. Overexpressed in the adenoma tissue of patients with Cushing's syndrome. May be involved in the molecular events of adrenocortical tumorigenesis by facilitating steroid synthesis and cell growth.

Protein type: EC 1.3.1.72; Membrane protein, integral; Lipid Metabolism - steroid biosynthesis; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cytoskeleton; membrane; endoplasmic reticulum; integral to membrane; nucleus; cytosol

Molecular Function: delta24(24-1) sterol reductase activity; UDP-N-acetylmuramate dehydrogenase activity; enzyme binding; peptide antigen binding; FAD binding; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; delta24-sterol reductase activity

Biological Process: skin development; tissue development; apoptosis; response to hormone stimulus; negative regulation of caspase activity; cholesterol biosynthetic process; negative regulation of cell proliferation; protein localization; amyloid precursor protein catabolic process; Ras protein signal transduction; male genitalia development; response to oxidative stress; cell cycle arrest; plasminogen activation; negative regulation of apoptosis

Disease: Desmosterolosis