Rabbit anti-Human DFFA Polyclonal Antibody | anti-DFFA antibody

Anti-DFFA Antibody

Cat. Num. AAA416354

• Gene Names: DFFA; DFF1; ICAD; DFF-45
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Protein A purified
• Synonyms: DFFA; Polyclonal Antibody; Anti-DFFA Antibody; anti-DFFA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DFFA antibody detects endogenous levels of DFFA protein. Not tested with other proteins
• Purity/Purification: Protein A purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 268

• Applicable Applications for anti-DFFA antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Recommended dilutions:; Peptide ELISA: 1:40,000; WB: 1:200 - 1:1000; Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Synthetic peptide conjugated to KLH
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Related Product Information for anti-DFFA antibody

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

References

Liu,X., Zou,H., Slaughter,C et,al DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 47132600
• NCBI GeneID: 1676
• NCBI Accession #: NP_998731.1
• NCBI GenBank Nucleotide #: NM_213566.1
• Molecular Weight: 29,411 Da
• NCBI Official Full Name: DNA fragmentation factor subunit alpha isoform 2
• NCBI Official Synonym Full Names: DNA fragmentation factor, 45kDa, alpha polypeptide
• NCBI Official Symbol: DFFA
• NCBI Official Synonym Symbols: DFF1; ICAD; DFF-45
• NCBI Protein Information: DNA fragmentation factor subunit alpha; DFF45; DNA fragmentation factor 45 kDa subunit; inhibitor of CAD
• UniProt Protein Name: DNA fragmentation factor subunit alpha
• Protein Family: DNA fragmentation factor
• UniProt Gene Name: DFFA
• UniProt Entry Name: DFFA_HUMAN

NCBI Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; lipid particle; cytosol; nucleus

Molecular Function: protein binding

Biological Process: apoptosis; positive regulation of apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis