Human Desmin ELISA Kit | DES elisa kit

Human Desmin ELISA Kit

Cat. Num. AAA2887277

• Gene Names: DES; CSM1; CSM2; LGMD2R
• Reactivity: Human
• Synonyms: Desmin; Human Desmin ELISA Kit; DES; DES elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human Desmin. No significant cross-reactivity or interference was observed.
• Sequence Length: 470

• Assay Type: Sandwich
• Detection Range: 78-5000 pg/mL
• Sensitivity: 39.1 pg/mL
• Intra-Assay CV: <=3.5%
• Inter-Assay CV: <=6.1%
• Recovery: 80%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DES elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Desmin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Desmin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Desmin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Desmin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Desmin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55749932
• NCBI GeneID: 1674
• NCBI Accession #: NP_001918.3
• NCBI GenBank Nucleotide #: NM_001927.3
• UniProt Accession #: P17661; Q15787; Q549R7; Q549R8; Q549R9; Q8IZR1; Q8IZR6; Q8NES2; Q8NEU6; Q8TAC4; Q8TCX2; Q8TD99
• Molecular Weight: 53,536 Da
• NCBI Official Full Name: desmin
• NCBI Official Synonym Full Names: desmin
• NCBI Official Symbol: DES
• NCBI Official Synonym Symbols: CSM1; CSM2; LGMD2R
• NCBI Protein Information: desmin
• UniProt Protein Name: Desmin
• Protein Family: Desmin
• UniProt Gene Name: DES
• UniProt Entry Name: DESM_HUMAN

NCBI Description

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]

Uniprot Description

desmin: a class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: cytosol; fascia adherens; intermediate filament; intermediate filament cytoskeleton; neuromuscular junction; sarcolemma; Z disc

Molecular Function: cytoskeletal protein binding; identical protein binding; protein binding; structural constituent of cytoskeleton

Biological Process: cytoskeleton organization and biogenesis; intermediate filament organization; muscle contraction; muscle filament sliding; regulation of heart contraction

Disease: Cardiomyopathy, Dilated, 1i; Muscular Dystrophy, Limb-girdle, Type 2r; Myopathy, Myofibrillar, 1; Scapuloperoneal Syndrome, Neurogenic, Kaeser Type