Goat Desmin ELISA Kit | Des elisa kit

Goat Desmin ELISA Kit

Cat. Num. AAA741323

• Gene Names: DES; CSM1; CSM2; LGMD2R
• Reactivity: Goat
• Synonyms: Desmin; Goat Desmin ELISA Kit; Des elisa kit

• Reactivity: Goat
• Specificity: This assay has high sensitivity and excellent specificity for detection of DES. No significant cross-reactivity or interference between DES and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DES and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Des elisa kit

Intended Uses: This DES ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat DES. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: DES ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DES antibody and an DES-HRP conjugate. The assay sample and buffer are incubated together with DES-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DES concentration since DES from samples and DES-HRP conjugate compete for the anti-DES antibody binding site. Since the number of sites is limited, as more sites are occupied by DES from the sample, fewer sites are left to bind DES-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DES concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Des elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 55749932
• NCBI GeneID: 1674
• NCBI Accession #: NP_001918.3
• NCBI GenBank Nucleotide #: NM_001927.3
• Molecular Weight: 53,536 Da
• NCBI Official Full Name: desmin
• NCBI Official Synonym Full Names: desmin
• NCBI Official Symbol: DES
• NCBI Official Synonym Symbols: CSM1; CSM2; LGMD2R
• NCBI Protein Information: desmin; mutant desmin p.K241E; intermediate filament protein
• UniProt Protein Name: Desmin
• Protein Family: Desmin
• UniProt Gene Name: DES
• UniProt Entry Name: DESM_HUMAN

NCBI Description

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]

Uniprot Description

desmin: a class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: intermediate filament; fascia adherens; neuromuscular junction; cytosol; Z disc; sarcolemma

Molecular Function: identical protein binding; protein binding; structural constituent of cytoskeleton; cytoskeletal protein binding

Biological Process: muscle contraction; cytoskeleton organization and biogenesis; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Dilated, 1i; Scapuloperoneal Syndrome, Neurogenic, Kaeser Type; Myopathy, Myofibrillar, 1; Muscular Dystrophy, Limb-girdle, Type 2r