Human Beta-defensin 126 ELISA Kit | DEFB126 elisa kit

Human Beta-defensin 126 ELISA Kit

Cat. Num. AAA765284

• Gene Names: DEFB126; DEFB26; hBD-26; C20orf8; DEFB-26; bA530N10.1
• Reactivity: Human
• Synonyms: Beta-defensin 126; Human Beta-defensin 126 ELISA Kit; DEFB126 (Beta-defensin 126)/Epididymal secretory protein/Beta-defensin 26/DEFB-26/Defensin; beta 126/C20orf8/DEFB26; DEFB126 elisa kit

• Reactivity: Human
• Sequence Length: 111

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for DEFB126 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human DEFB126 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human DEFB126 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human DEFB126 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human DEFB126 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 13624333
• NCBI GeneID: 81623
• NCBI Accession #: NP_112193.1
• NCBI GenBank Nucleotide #: NM_030931.3
• Molecular Weight: 12,174 Da
• NCBI Official Full Name: beta-defensin 126 preproprotein
• NCBI Official Synonym Full Names: defensin beta 126
• NCBI Official Symbol: DEFB126
• NCBI Official Synonym Symbols: DEFB26; hBD-26; C20orf8; DEFB-26; bA530N10.1
• NCBI Protein Information: beta-defensin 126
• UniProt Protein Name: Beta-defensin 126
• Protein Family: Beta-defensin
• UniProt Gene Name: DEFB126
• UniProt Synonym Gene Names: C20orf8; DEFB26; DEFB-26; ESP13.2
• UniProt Entry Name: DB126_HUMAN

NCBI Description

Defensins are cysteine-rich cationic polypeptides that are important in the immunologic response to invading microorganisms. The antimicrobial protein encoded by this gene is secreted and is a member of the beta defensin protein family. Beta defensin genes are found in several clusters throughout the genome, with this gene mapping to a cluster at 20p13. The encoded protein is highly similar to an epididymal-specific secretory protein (ESP13.2) from cynomolgus monkey. Mutation of this gene is associated with impaired sperm function. [provided by RefSeq, Nov 2014]

Uniprot Description

defensin, beta 26: Has antibacterial activity (Potential). Belongs to the beta-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: cell surface; extracellular region; glycocalyx

Biological Process: defense response to bacterium; innate immune response