Human defensin, beta 104A ELISA Kit | DEFB104A elisa kit

Human Beta-defensin 104, DEFB104A ELISA Kit

Cat. Num. AAA913953

• Gene Names: DEFB104B; BD-4; hBD-4; DEFB-4
• Reactivity: Human
• Synonyms: defensin; beta 104A; Human Beta-defensin 104; DEFB104A ELISA Kit; Human Beta-defensin 104 (DEFB104A) ELISA kit; BD-4; DEFB-4; DEFB104; DEFB4; MGC118942; MGC118944; MGC118945; hBD-4; beta 4; DEFB104A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human DEFB104A. No significant cross-reactivity or interference between human DEFB104A and analogues was observed.
• Sequence Length: 72

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml.
• Sensitivity: The minimum detectable dose of human DEFB104A is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DEFB104A elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFB104A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFB104A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFB104A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFB104A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 103485137
• NCBI GeneID: 503618
• NCBI Accession #: NP_001035792.1
• NCBI GenBank Nucleotide #: NM_001040702.1
• UniProt Accession #: Q8WTQ1; Q496I2; Q496I3; Q496I4
• Molecular Weight: 8,526 Da
• NCBI Official Full Name: beta-defensin 104
• NCBI Official Synonym Full Names: defensin, beta 104B
• NCBI Official Symbol: DEFB104B
• NCBI Official Synonym Symbols: BD-4; hBD-4; DEFB-4
• NCBI Protein Information: beta-defensin 104; beta-defensin 4; defensin, beta 104
• UniProt Protein Name: Beta-defensin 104
• Protein Family: Beta-defensin
• UniProt Gene Name: DEFB104A
• UniProt Synonym Gene Names: DEFB104; DEFB4; DEFB104B; BD-4; DEFB-4; hBD-4
• UniProt Entry Name: D104A_HUMAN

NCBI Description

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin, beta 104, DEFB104A and DEFB104B, in head-to-head orientation. This gene, DEFB104B, represents the more telomeric copy. [provided by RefSeq, Jul 2008]

Uniprot Description

defensin, beta 4: Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103. Belongs to the beta-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: cell surface; extracellular region

Biological Process: defense response to bacterium; innate immune response