Human Beta-defensin 1 ELISA Kit | DEFB1 elisa kit

Human Beta-defensin 1 ELISA Kit

Cat. Num. AAA762113

• Gene Names: DEFB1; BD1; HBD1; DEFB-1; DEFB101
• Reactivity: Human
• Synonyms: Beta-defensin 1; Human Beta-defensin 1 ELISA Kit; DEFB1/Beta-defensin 1/BD1/HBD1/BD-1/hBD-1/Defensin Beta 1; DEFB1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DEFB1. No significant cross-reactivity or interference between DEFB1 and analogues was observed.
• Sequence Length: 68

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for DEFB1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4885181
• NCBI GeneID: 1672
• NCBI Accession #: NP_005209.1
• NCBI GenBank Nucleotide #: NM_005218.3
• Molecular Weight: 7,420 Da
• NCBI Official Full Name: beta-defensin 1 preproprotein
• NCBI Official Synonym Full Names: defensin beta 1
• NCBI Official Symbol: DEFB1
• NCBI Official Synonym Symbols: BD1; HBD1; DEFB-1; DEFB101
• NCBI Protein Information: beta-defensin 1
• UniProt Protein Name: Beta-defensin 1
• Protein Family: Beta-defensin
• UniProt Gene Name: DEFB1
• UniProt Synonym Gene Names: BD1; HBD1; BD-1; hBD-1
• UniProt Entry Name: DEFB1_HUMAN

NCBI Description

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq, Jul 2008]

Uniprot Description

defensin, beta 1: Has bactericidal activity. Belongs to the beta-defensin family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular region; extracellular space; Golgi lumen

Biological Process: acute inflammatory response; antibacterial humoral response; chemotaxis; defense response to Gram-positive bacterium; G-protein coupled receptor protein signaling pathway; immune response; innate immune response; innate immune response in mucosa; response to bacterium; response to testosterone stimulus