Human Defensin-5 ELISA Kit | DEFA5 elisa kit

Human Defensin-5 ELISA Kit

Cat. Num. AAA2887546

• Gene Names: DEFA5; DEF5; HD-5
• Reactivity: Human
• Synonyms: Defensin-5; Human Defensin-5 ELISA Kit; Defensin; alpha 5; DEFA5; DEF5; HD5(20-94); DEFA5 elisa kit

• Reactivity: Human
• Specificity: Recombinant and nature Human Defensin-5
• Sequence Length: 94

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 15.6-1000 pg/mL
• Sensitivity: <8.9 pg/mL
• Intra-assay Precision: Intra-Assay CV: <=7.1%
• Inter-assay Precision: Inter-Assay CV: <=9.2%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DEFA5 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10337585
• NCBI GeneID: 1670
• NCBI Accession #: NP_066290.1
• NCBI GenBank Nucleotide #: NM_021010.2
• UniProt Accession #: Q01523; Q3KNV2; A0JDY6
• Molecular Weight: 10,071 Da
• NCBI Official Full Name: defensin-5 preproprotein
• NCBI Official Synonym Full Names: defensin alpha 5
• NCBI Official Symbol: DEFA5
• NCBI Official Synonym Symbols: DEF5; HD-5
• NCBI Protein Information: defensin-5
• UniProt Protein Name: Defensin-5
• Protein Family: Defensin
• UniProt Gene Name: DEFA5
• UniProt Synonym Gene Names: DEF5
• UniProt Entry Name: DEF5_HUMAN

NCBI Description

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several of the alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 5, is highly expressed in the secretory granules of Paneth cells of the ileum. [provided by RefSeq, Oct 2014]

Uniprot Description

DEFA5: Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing. Belongs to the alpha-defensin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular region; extracellular space; Golgi lumen; transport vesicle

Biological Process: antibacterial humoral response; defense response to fungus; innate immune response; killing of cells of another organism