Defensin Alpha 3, Neutrophil Specific (DEFa3) Recombinant Protein | DEFa3 recombinant protein

Recombinant Defensin Alpha 3, Neutrophil Specific (DEFa3)

Cat. Num. AAA2010173

• Gene Names: DEFA3; HP3; DEF3; HNP3; HP-3; HNP-3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation, Immunoprecipitation
• Purity: > 95%
• Synonyms: Defensin Alpha 3; Neutrophil Specific (DEFa3); Recombinant Defensin Alpha 3; DEFa3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-E PLQARADEVA AAPEQIAADI PEVVVSLAWD ESLAPKHPGS RKNMDCYCRI PACIAGERRY GTCIYQGRLW AFCC
• Sequence Length: 94

• Applicable Applications for DEFa3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, Reporter Assays, Purification, Amine Reactive Labeling.
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Pro21~Cys94
• Homology: Mouse 34%, rat 33%
• Tissue Specificity: Lung, spleen, pancreas.
• Subcellular Location: Secreted.
• Traits: Freeze-dried powder
• Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA,1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Predicted isoelectric point: 6.4
• Predicted Molecular Mass: 9.7kDa
• Accurate Molecular Mass: 11kDa as determined by SDS-PAGE reducing conditions.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 4885179
• NCBI GeneID: 1668
• NCBI Accession #: NP_005208.1
• NCBI GenBank Nucleotide #: NM_005217.3
• UniProt Accession #: P59666; P11479; Q14125
• NCBI Official Full Name: neutrophil defensin 3 preproprotein
• NCBI Official Synonym Full Names: defensin, alpha 3, neutrophil-specific
• NCBI Official Symbol: DEFA3
• NCBI Official Synonym Symbols: HP3; DEF3; HNP3; HP-3; HNP-3
• NCBI Protein Information: neutrophil defensin 3; neutrophil peptide 3; defensin 3, neutrophil-specific
• UniProt Protein Name: Neutrophil defensin 3
• Protein Family: Alpha-defensin
• UniProt Gene Name: DEFA3
• UniProt Synonym Gene Names: DEF3; HP-3; HP3; HP-2; HP2
• UniProt Entry Name: DEF3_HUMAN

NCBI Description

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 3, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 1 by only one amino acid. This gene and the gene encoding defensin, alpha 1 are both subject to copy number variation. [provided by RefSeq, Oct 2014]

Uniprot Description

defensin, alpha 3: Defensin 2 and defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; defense response to virus; defense response to fungus