Typical Testing Data/Standard Curve (for reference only)
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Human D-aspartate oxidase ELISA Kit | DDO elisa kit
Human D-aspartate oxidase ELISA Kit
Gene Names
DDO; DASOX; DDO-1; DDO-2
Reactivity
Human
Synonyms
D-aspartate oxidase; Human D-aspartate oxidase ELISA Kit; DDO/D-aspartate oxidase/DASOX/DDO-1/DDO-2; DDO elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of DDO. No significant cross-reactivity or interference between DDO and analogues was observed.
Sequence Length
341
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for DDO elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33,980 Da
NCBI Official Full Name
D-aspartate oxidase
NCBI Official Synonym Full Names
D-aspartate oxidase
NCBI Official Symbol
DDO
NCBI Official Synonym Symbols
DASOX; DDO-1; DDO-2
NCBI Protein Information
D-aspartate oxidase
UniProt Protein Name
D-aspartate oxidase
Protein Family
UniProt Gene Name
DDO
UniProt Synonym Gene Names
DASOX; DDO
UniProt Entry Name
OXDD_HUMAN
NCBI Description
The protein encoded by this gene is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate.
Research Articles on DDO
Similar Products
Product Notes
The Human DDO ddo (Catalog #AAA763196) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA763196 ELISA Kit recognizes Human DDO. It is sometimes possible for the material contained within the vial of "D-aspartate oxidase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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