Typical Testing Data/Standard Curve (for reference only)
Human Aromatic L amino acid decarboxylase (DDC) ELISA Kit | DDC elisa kit
Human Aromatic L amino acid decarboxylase (DDC) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: DDC ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DDC antibody and an DDC-HRP conjugate. The assay sample and buffer are incubated together with DDC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDC concentration since DDC from samples and DDC-HRP conjugate compete for the anti-DDC antibody binding site. Since the number of sites is limited, as more sites are occupied by DDC from the sample, fewer sites are left to bind DDC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDC concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]
Uniprot Description
DDC: Catalyzes the decarboxylation of L-3,4- dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Homodimer. Belongs to the group II decarboxylase family.
Protein type: Amino Acid Metabolism - histidine; Lyase; Amino Acid Metabolism - phenylalanine; EC 4.1.1.28; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - tryptophan; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 7p12.2
Cellular Component: synaptic vesicle; cell soma; axon; cytosol
Molecular Function: amino acid binding; aromatic-L-amino-acid decarboxylase activity; protein domain specific binding; protein binding; enzyme binding; pyridoxal phosphate binding
Biological Process: circadian rhythm; amino acid metabolic process; catecholamine biosynthetic process; indolalkylamine biosynthetic process; multicellular organismal aging; isoquinoline alkaloid metabolic process; dopamine biosynthetic process; serotonin biosynthetic process; phytoalexin metabolic process; synaptic vesicle amine transport; response to pyrethroid
Disease: Aromatic L-amino Acid Decarboxylase Deficiency
Research Articles on DDC
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Product Notes
The Human DDC ddc (Catalog #AAA7238872) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7238872 ELISA Kit recognizes Human DDC. It is sometimes possible for the material contained within the vial of "Aromatic L amino acid decarboxylase (DDC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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