DNA damage-binding protein 1 (DDB1) Recombinant Protein | DDB1 recombinant protein

Recombinant Bovine DNA damage-binding protein 1 (DDB1) , partial

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA damage-binding protein 1 (DDB1); Recombinant Bovine DNA damage-binding protein 1 (DDB1); partial; DDB1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1140

• Species: Bovine
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for DDB1 recombinant protein

This gene encodes the large subunit of DNA damage-binding protein which is a heterodimer composed of a large and a small subunit. This protein functions in nucleotide-excision repair. Its defective activity causes the repair defect in the patients with xeroderma pigmentosum complementation group E (XPE). However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients.

NCBI and Uniprot Product Information

• NCBI GI #: 122692537
• NCBI GeneID: 511951
• NCBI Accession #: NP_001073731.1
• NCBI GenBank Nucleotide #: NM_001080262.1
• UniProt Accession #: A1A4K3
• Molecular Weight: 126,930 Da
• NCBI Official Full Name: DNA damage-binding protein 1
• NCBI Official Synonym Full Names: damage specific DNA binding protein 1
• NCBI Official Symbol: DDB1
• NCBI Protein Information: DNA damage-binding protein 1
• UniProt Protein Name: DNA damage-binding protein 1
• Protein Family: DNA damage-binding protein
• UniProt Gene Name: DDB1

Uniprot Description

Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2 ().