Scavenger mRNA-decapping enzyme DcpS (DCPS) Recombinant Protein | DCPS recombinant protein

Recombinant Human Scavenger mRNA-decapping enzyme DcpS (DCPS)

Cat. Num. AAA1460460

• Gene Names: DCPS; ARS; DCS1; HSL1; HINT5; HINT-5; HSPC015
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Scavenger mRNA-decapping enzyme DcpS (DCPS); Recombinant Human Scavenger mRNA-decapping enzyme DcpS (DCPS); DCPS recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-337aa; Full Length of Mature Protein
• Sequence: ADAAPQLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASGDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRNITLPHLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQQLDDLYLIAICHRRGIRSLRDLTPEHLPLLRNILHQGQEAILQRYRMKGDHLRVYLHYLPSYYHLHVHFTALGFEAPGSGVERAHLLAEVIENLECDPRHYQQRTLTFALRADDPLLKLLQEAQQS

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 7661734
• NCBI GeneID: 28960
• NCBI Accession #: NP_054745.1
• NCBI GenBank Nucleotide #: NM_014026.5
• UniProt Accession #: Q96C86; Q8NHL8; Q9Y2S5
• Molecular Weight: 38,609 Da
• NCBI Official Full Name: m7GpppX diphosphatase isoform 2
• NCBI Official Synonym Full Names: decapping enzyme, scavenger
• NCBI Official Symbol: DCPS
• NCBI Official Synonym Symbols: ARS; DCS1; HSL1; HINT5; HINT-5; HSPC015
• NCBI Protein Information: m7GpppX diphosphatase
• UniProt Protein Name: m7GpppX diphosphatase
• Protein Family: m7GpppX diphosphatase
• UniProt Gene Name: DCPS
• UniProt Synonym Gene Names: DCS1; HINT5; HINT-5

NCBI Description

This gene encodes a member of the histidine triad family of pyrophosphatases that removes short mRNA fragments containing the 5′ mRNA cap structure, which appear in the 3′ → 5′ mRNA decay pathway, following deadenylation and exosome-mediated turnover. This enzyme hydrolyzes the triphosphate linkage of the cap structure (7-methylguanosine nucleoside triphosphate) to yield 7-methylguanosine monophosphate and nucleoside diphosphate. It protects the cell from the potentially toxic accumulation of these short, capped mRNA fragments, and regulates the activity of other cap-binding proteins, which are inhibited by their accumulation. It also acts as a transcript-specific modulator of pre-mRNA splicing and microRNA turnover. [provided by RefSeq, Apr 2017]

Uniprot Description

Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP (PubMed:22985415). May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP (PubMed:14523240). Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre-mRNAs. Inhibits activation-induced cell death.