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Typical Testing Data/Standard Curve (for reference only)

Mouse decorin ELISA Kit | DCN elisa kit

Mouse Decorin, DCN ELISA Kit

Gene Names
Dcn; DC; PG40; PGII; PGS2; mDcn; DSPG2; SLRR1B
Reactivity
Mouse
Synonyms
decorin; Mouse Decorin; DCN ELISA Kit; Mouse Decorin (DCN) ELISA kit; CSCD; DSPG2; PG40; PGII; PGS2; SLRR1B; bone proteoglycan II; decorin proteoglycan; dermatan sulphate proteoglycans II; proteoglycan core protein; small leucine-rich protein 1B; DCN elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse DCN. No significant cross-reactivity or interference between mouse DCN and analogues was observed.
Sequence Length
354
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich (Quantitative)
Detection Range
78 pg/ml -5000 pg/ml.
Sensitivity
19.5 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DCN elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DCN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DCN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCN bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39,809 Da
NCBI Official Full Name
decorin preproprotein
NCBI Official Synonym Full Names
decorin
NCBI Official Symbol
Dcn
NCBI Official Synonym Symbols
DC; PG40; PGII; PGS2; mDcn; DSPG2; SLRR1B
NCBI Protein Information
decorin; bone proteoglycan II
UniProt Protein Name
Decorin
Protein Family
UniProt Gene Name
Dcn
UniProt Entry Name
PGS2_MOUSE

NCBI Description

This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded preproprotein is proteolytically processed to generate a mature protein product, which is secreted into the extracellular space to regulate collagen fibril assembly. Homozygous knockout mice for this gene exhibit enhanced tumorigenesis in a liver cancer model, and defects in collagen fibrils, leading to weakened skin and tendons. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix; Motility/polarity/chemotaxis

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region; collagen type VI

Molecular Function: collagen binding; glycosaminoglycan binding; extracellular matrix binding; protein N-terminus binding

Biological Process: peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan

Research Articles on DCN

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Product Notes

The Mouse DCN dcn (Catalog #AAA933682) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA933682 ELISA Kit recognizes Mouse DCN. It is sometimes possible for the material contained within the vial of "decorin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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