Protein artemis (Dclre1c) Recombinant Protein | Dclre1c recombinant protein

Recombinant Mouse Protein artemis (Dclre1c)

Cat. Num. AAA1350215

• Gene Names: Dclre1c; Art; Snm1l; AI661365; 9930121L06Rik
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Protein artemis (Dclre1c); Recombinant Mouse Protein artemis (Dclre1c); Dclre1c recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-705, Full length protein
• Sequence: MSSFQGQMAEYPTISIDRFDRENLKARAYFLSHCHKDHMKGLRAPSLKRRLECSLKVFLYCSPVTKELLLTSPKYRFWENRIITIEIETPTQISLVDEASGEKEEVVVTLLPAGHCPGSVMFLFQGSNGTVLYTGDFRLAKGEASRMELLHSGGRVKDIQSVYLDTTFCDPRFYQIPSREQCLRGILELVRSWVTRSPHHVVWLNCKAAYGYEYLFTNLSEELGVQVHVDKLDMFKNMPDILHHLTTDRNTQIHACRHPKAEECFQWNKLPCGITSQNKTALHTISIKPSTMWFGERTRKTNVIVRTGESSYRACFSFHSSFSEIKDFLSYICPVNVYPNVIPVGLTVDKVMDVLKPLCRSPQSVEPKYKPLGKLKRARTIHLDSEEDDDLFDDPLPTPLRHKVPYQLTLQPELFSMKALPLDQPELRQSPGGCKAESVWSPSLANFIDCEESNSDSGEELETPPPSLQGGLGPSTLVQQNADPDVDIPQWEVFFKRRDEITGECLEHLPSSIETGGSQSPKLCSDSPKLCSDSPKLCSDSDGDSTHISSQNSSQSTHITDQGSQGWDSQCDTVLLSSQEKSGGDSTSLNKGAYKPKLKESISASQIEQDALCPQDTHCDLKSRAEVNGAPCLVELDTLSGRKSPPEKTLLSSTRADSQSSSDFEIPSTPEAELPTPEHLQCLYRKLATGQSIVVEKRKCSLLDS
• Sequence Length: 705

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Dclre1c recombinant protein

This gene encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The protein has single-strand-specific 5 -3 exonuclease activity; it also exhibits endonuclease activity on 5 and 3 overhangs and hairpins when complexed with protein kinase, DNA-activated, catalytic polypeptide. Mutations in this gene cause Athabascan-type severe combined immunodeficiency (SCIDA).

NCBI and Uniprot Product Information

• NCBI GI #: 158966682
• NCBI GeneID: 227525
• NCBI Accession #: NP_001103684.1
• NCBI GenBank Nucleotide #: NM_001110214.1
• UniProt Accession #: Q8K4J0; Q8BG72; Q8BTT1; A2AJG6; A2AJG7; A2AJG8
• Molecular Weight: 54,281 Da
• NCBI Official Full Name: protein artemis isoform 3
• NCBI Official Synonym Full Names: DNA cross-link repair 1C
• NCBI Official Symbol: Dclre1c
• NCBI Official Synonym Symbols: Art; Snm1l; AI661365; 9930121L06Rik
• NCBI Protein Information: protein artemis
• UniProt Protein Name: Protein artemis
• Protein Family: Protein artemis
• UniProt Gene Name: Dclre1c
• UniProt Synonym Gene Names: Art; Snm1l; mArt

NCBI Description

This gene encodes a member of the SNM1 family of nucleases and is involved in V(D)J recombination and DNA repair. This protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins. The protein also functions in the regulation of the cell cycle in response to DNA damage. Homozygous knockout mice for this gene exhibit severe combined immunodeficiency with sensitivity to ionizing radiation. Mutations in this gene in humans can cause Athabascan-type severe combined immunodeficiency (SCIDA) and Omenn syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]

Uniprot Description

Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein likely exhibits single-strand specific 5'-3' exonuclease activity in isolation, and may acquire endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity may be required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.