Chicken Dendritic cell Associated C type Lectin 1 ELISA Kit | DCAL-2 elisa kit

Chicken Dendritic cell Associated C type Lectin 1 ELISA Kit

Cat. Num. AAA740951

• Gene Names: CLEC7A; BGR; CANDF4; DECTIN1; CLECSF12
• Reactivity: Chicken
• Synonyms: Dendritic cell Associated C type Lectin 1; Chicken Dendritic cell Associated C type Lectin 1 ELISA Kit; DCAL-2 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This DCAL-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken DCAL-2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DCAL-2 elisa kit

Principle of the Assay: DCAL-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DCAL-2 antibody and an DCAL-2-HRP conjugate. The assay sample and buffer are incubated together with DCAL-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DCAL-2 concentration since DCAL-2 from samples and DCAL-2-HRP conjugate compete for the anti-DCAL-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by DCAL-2 from the sample, fewer sites are left to bind DCAL-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DCAL-2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for DCAL-2 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 13649708
• NCBI GeneID: 64581
• UniProt Accession #: Q9BXN2; Q6IPS7; Q96D32; Q96DR9; Q96LD3; Q96PA5; Q96PA6; Q96PA7; B2R861; B7Z494; B7Z5A9; B7Z5B9; Q68D78; Q6UWR2; Q96PA4
• Molecular Weight: 27,627 Da
• NCBI Official Full Name: dendritic cell-associated C-type lectin-1
• NCBI Official Synonym Full Names: C-type lectin domain family 7, member A
• NCBI Official Symbol: CLEC7A
• NCBI Official Synonym Symbols: BGR; CANDF4; DECTIN1; CLECSF12
• NCBI Protein Information: C-type lectin domain family 7 member A; dectin-1; beta-glucan receptor; lectin-like receptor 1; DC-associated C-type lectin 1; C-type lectin superfamily member 12; dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12
• UniProt Protein Name: C-type lectin domain family 7 member A
• UniProt Gene Name: CLEC7A
• UniProt Synonym Gene Names: BGR; CLECSF12; DECTIN1; DC-associated C-type lectin 1; Dectin-1
• UniProt Entry Name: CLC7A_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

Uniprot Description

dectin-1: Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Up-regulated during differentiation from monocytes into dendritic cells. Isoform 5 interacts with RANBP9. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2

Cellular Component: cytoplasm; integral to membrane; plasma membrane

Molecular Function: metal ion binding; carbohydrate binding; MHC protein binding; pattern recognition receptor activity

Biological Process: T cell activation; innate immune response; carbohydrate mediated signaling; cell recognition; phagocytosis, recognition; pattern recognition receptor signaling pathway; defense response to protozoan; inflammatory response

Disease: Candidiasis, Familial, 4; Aspergillosis, Susceptibility To