Protein VPRBP (VPRBP) Recombinant Protein | VPRBP recombinant protein

Recombinant Human Protein VPRBP (VPRBP) , partial

Cat. Num. AAA1484948

• Gene Names: DCAF1; RIP; VPRBP
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Protein VPRBP (VPRBP); Recombinant Human Protein VPRBP (VPRBP); partial; VPRBP recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 141-500aa; Partial(Protein kinase-like Region)
• Sequence: QPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLED

• Species: Human
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 284813529
• NCBI GeneID: 9730
• NCBI Accession #: NP_001165375.1
• NCBI GenBank Nucleotide #: NM_001171904.1
• UniProt Accession #: Q9Y4B6; Q2YD74; Q8TBD9; Q9HCA1; Q9UG37
• Molecular Weight: 118,235 Da
• NCBI Official Full Name: DDB1- and CUL4-associated factor 1 isoform 2
• NCBI Official Synonym Full Names: DDB1 and CUL4 associated factor 1
• NCBI Official Symbol: DCAF1
• NCBI Official Synonym Symbols: RIP; VPRBP
• NCBI Protein Information: DDB1- and CUL4-associated factor 1; protein VPRBP
• UniProt Protein Name: DDB1- and CUL4-associated factor 1
• Protein Family: Protein
• UniProt Gene Name: DCAF1
• UniProt Synonym Gene Names: VprBP

Uniprot Description

Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification. Probable substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, named CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, which mediates ubiquitination and proteasome-dependent degradation of proteins such as NF2. Involved in the turnover of methylated proteins: recognizes and binds methylated proteins via its chromo domain, leading to ubiquitination of target proteins by the RBX1-DDB1-DCAF1/VPRBP complex (PubMed:23063525). The CUL4A-RBX1-DDB1-DCAF1/VPRBP complex is also involved in B-cell development: DCAF1 is recruited by RAG1 to ubiquitinate proteins, leading to limit error-prone repair during V(D)J recombination. Also part of the EDVP complex, an E3 ligase complex that mediates ubiquitination of proteins such as TERT, leading to TERT degradation and telomerase inhibition (PubMed:23362280). Also acts as an atypical serine/threonine-protein kinase that specifically mediates phosphorylation of 'Thr-120' of histone H2A (H2AT120ph) in a nucleosomal context, thereby repressing transcription. H2AT120ph is present in the regulatory region of many tumor suppresor genes, down-regulates their transcription and is present at high level in a number of tumors (PubMed:24140421). Involved in JNK-mediated apoptosis during cell competition process via its interaction with LLGL1 and LLGL2 (PubMed:20644714).