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Canine D-Lactate Dehydrogenase ELISA Kit | D-LDH elisa kit

Canine D-Lactate Dehydrogenase ELISA Kit

Reactivity
Canine
Synonyms
D-Lactate Dehydrogenase; Canine D-Lactate Dehydrogenase ELISA Kit; D-LDH elisa kit
Ordering
For Research Use Only!
Reactivity
Canine
Specificity
No significant cross-reactivity or interference between Canine D-LDH and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type
Sandwich
Detection Range
6.25 U/L – 200 U/L.
Sensitivity
1.0 U/L.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated D-LDH concentrations in Canine serum, plasma and other body fluids. Using Purified Canine D-LDH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add D-LDH and D-LDH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of D-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for D-LDH elisa kit
Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated D-LDH concentrations in Canine serum, plasma and other body fluids. Using Purified Canine D-LDH antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add D-LDH and D-LDH antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of D-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
36,682 Da
NCBI Official Full Name
D-lactate dehydrogenase
NCBI Official Symbol
SACOL2535
NCBI Protein Information
D-lactate dehydrogenase
UniProt Protein Name
D-lactate dehydrogenase
Protein Family
UniProt Gene Name
ldhD
UniProt Synonym Gene Names
ddh; D-LDH
UniProt Entry Name
LDHD_STAAC

Uniprot Description

Catalytic activity: (R)-lactate + NAD+ = pyruvate + NADH.

Sequence similarities: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Similar Products

Product Notes

The Canine D-LDH ldhd (Catalog #AAA017416) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA017416 ELISA Kit recognizes Canine D-LDH. It is sometimes possible for the material contained within the vial of "D-Lactate Dehydrogenase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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