Mouse Cyclophilin B ELISA Kit | CYPB elisa kit

Mouse Cyclophilin B ELISA Kit

Cat. Num. AAA761239

• Gene Names: PPIB; OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
• Reactivity: Mouse
• Synonyms: Cyclophilin B; Mouse Cyclophilin B ELISA Kit; CYPB/CYP-S1/PPIB/S-cyclophilin/SCYLP/cyclophilin-like protein//CYPBEC 5.2.1.8/CYP-S1/MGC2224/OI9MGC14109/peptidyl-prolyl cis-trans isomerase B/peptidylprolyl isomerase B (cyclophilin B)/PPIase B/Rotamase B/S-cyclophilin/SCYLP; CYPB elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CYPB. No significant cross-reactivity or interference between CYPB and analogues was observed.
• Sequence Length: 208

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 125-8000pg/ml
• Sensitivity: 75pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CYPB were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CYPB were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYPB elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CYPB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CYPB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CYPB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CYPB can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 181335
• NCBI GeneID: 5479
• Molecular Weight: 23,743 Da
• NCBI Official Full Name: cyclophilin B
• NCBI Official Synonym Full Names: peptidylprolyl isomerase B
• NCBI Official Symbol: PPIB
• NCBI Official Synonym Symbols: OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase B
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase B
• Protein Family: Bifunctional cytochrome P450/NADPH--P450 reductase
• UniProt Gene Name: PPIB
• UniProt Synonym Gene Names: CYPB; PPIase B; SCYLP
• UniProt Entry Name: PPIB_HUMAN

NCBI Description

The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009]

Uniprot Description

PPIB: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9). OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients. Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.

Protein type: Secreted, signal peptide; Cyclophilin; Isomerase; EC 5.2.1.8; RNA-binding; Chaperone; Secreted

Chromosomal Location of Human Ortholog: 15q21-q22

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; focal adhesion; melanosome; membrane; nucleus; perinuclear region of cytoplasm; smooth endoplasmic reticulum

Molecular Function: collagen binding; peptide binding; peptidyl-prolyl cis-trans isomerase activity; protein binding; protein complex binding; unfolded protein binding

Biological Process: extracellular matrix organization and biogenesis; positive regulation of multicellular organism growth; protein peptidyl-prolyl isomerization; protein stabilization

Disease: Osteogenesis Imperfecta, Type Ix