Human Cytochrome P450 2C9 ELISA Kit | CYP2C9 elisa kit

Human Cytochrome P450 2C9 ELISA Kit

Cat. Num. AAA765391

• Gene Names: CYP2C9; CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
• Reactivity: Human
• Synonyms: Cytochrome P450 2C9; Human Cytochrome P450 2C9 ELISA Kit; CYP2C9/Cytochrome P450 2C9/Cytochrome P450 MP-4/Cytochrome P-450MP/CYPIIC9/ (S)-limonene 7-monooxygenase/S-mephenytoin 4-hydroxylase/Cytochrome P450 PB-1/Cytochrome P450 MP-8/ (R)-limonene 6-monooxygenase/ (S)-limonene 6-monooxygenase/CYP2C10/CPC9/CYP2C; CYP2C9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CYP2C9. No significant cross-reactivity or interference between CYP2C9 and analogues was observed.
• Sequence Length: 490

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP2C9 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 13699818
• NCBI GeneID: 1559
• NCBI Accession #: NP_000762.2
• NCBI GenBank Nucleotide #: NM_000771.3
• Molecular Weight: 18,005 Da
• NCBI Official Full Name: cytochrome P450 2C9
• NCBI Official Synonym Full Names: cytochrome P450 family 2 subfamily C member 9
• NCBI Official Symbol: CYP2C9
• NCBI Official Synonym Symbols: CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
• NCBI Protein Information: cytochrome P450 2C9
• UniProt Protein Name: Cytochrome P450 2C9
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2C9
• UniProt Synonym Gene Names: CYP2C10
• UniProt Entry Name: CP2C9_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2C9: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S- warfarin, diclofenac, phenytoin, tolbutamide and losartan. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - arachidonic acid; Cofactor and Vitamin Metabolism - retinol; Lipid Metabolism - linoleic acid; EC 1.14.14.1; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Oxidoreductase; Secondary Metabolites Metabolism - limonene and pinene degradation; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: (S)-limonene 6-monooxygenase activity; (S)-limonene 7-monooxygenase activity; arachidonic acid epoxygenase activity; cholesterol 25-hydroxylase activity; drug binding; heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity; steroid hydroxylase activity

Biological Process: amide metabolic process; arachidonic acid metabolic process; drug catabolic process; drug metabolic process; epoxygenase P450 pathway; exogenous drug catabolic process; monocarboxylic acid metabolic process; monoterpenoid metabolic process; steroid metabolic process; urea metabolic process; xenobiotic metabolic process

Disease: Coumarin Resistance