anti-Human Cytochrome P450 2C19 Antibody | anti-CYP2C19 antibody

Cytochrome P450 2C19 Antibody

Cat. Num. AAA8509823

• Gene Names: CYP2C19; CPCJ; CYP2C; P450C2C; CYPIIC17; CYPIIC19; P450IIC19
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Cytochrome P450 2C19; Cytochrome P450 2C19 Antibody; EC 1.14.13.80; (R)-limonene 6-monooxygenase; EC 1.14.13.48; (S)-limonene 6-monooxygenase; EC 1.14.13.49; (S)-limonene 7-monooxygenase; CYPIIC19; P450-11A; Mephenytoin 4-hydroxylase; CYPIIC17; P450-254C; CP2CJ; anti-CYP2C19 antibody

• Reactivity: Human
• Specificity: Cytochrome P450 2C19 antibody detects endogenous levels of total Cytochrome P450 2C19 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 490

• Applicable Applications for anti-CYP2C19 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized peptide derived from C-terminal of human Cytochrome P450 2C19.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, using Cytochrome P450 2C19 antibody.)

References

Romkes M., Biochemistry 30:3247-3255(1991). Deloukas P., Nature 429:375-381(2004). Miyazawa M., Drug Metab. Dispos. 30:602-607(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 4503219
• NCBI GeneID: 1557
• NCBI Accession #: NP_000760.1
• NCBI GenBank Nucleotide #: NM_000769.3
• UniProt Accession #: P33261; P33259; Q8WZB1; Q8WZB2; Q9UCD4
• Molecular Weight: 55,931 Da
• NCBI Official Full Name: cytochrome P450 2C19
• NCBI Official Synonym Full Names: cytochrome P450 family 2 subfamily C member 19
• NCBI Official Symbol: CYP2C19
• NCBI Official Synonym Symbols: CPCJ; CYP2C; P450C2C; CYPIIC17; CYPIIC19; P450IIC19
• NCBI Protein Information: cytochrome P450 2C19
• UniProt Protein Name: Cytochrome P450 2C19
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2C19

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, omeprazole, diazepam and some barbiturates. Polymorphism within this gene is associated with variable ability to metabolize mephenytoin, known as the poor metabolizer and extensive metabolizer phenotypes. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq, Jul 2008]

Uniprot Description

Responsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol, citalopram and imipramine.CautionP450-254C was originally listed as a separate gene (CYP2C17). Resequencing demonstrated that it is not a separate gene, but a chimera. The 5'-portion corresponds to a partial 2C18 clone, and the 3'-portion corresponds to a partial 2C19 clone.