Cytochrome P450 1A1 (CYP1A1) Recombinant Protein | CYP1A1 recombinant protein

Recombinant Cytochrome P450 1A1 (CYP1A1)

Cat. Num. AAA2018442

• Gene Names: CYP1A1; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cytochrome P450 1A1 (CYP1A1); Recombinant Cytochrome P450 1A1 (CYP1A1); CYP1A1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MLFPISMSAT EFLLASVIFC LVFWVIRASR PQVPKGLKNP PGPWGWPLIG HMLTLGKNPH LALSRMSQQY GDVLQIRIGS TPVVVLSGLD TIRQALVRQG DDFKGRPDLY TFTLISNGQS MSFSPDSGPV WAARRRLAQN GLKSFSIASD PASSTSCYLE EHVSKEAEVL ISTLQELMAG PGHFNPYRYV VVSVTNVICAICFGRRYDHN HQELLSLVNL NNNFGEVVGS GNPADFIPIL RYLPNPSLNA
• Sequence Length: 512

• Applicable Applications for CYP1A1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Met1~Ala250 (Accession # P04798) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4503199
• NCBI GeneID: 1543
• NCBI Accession #: NP_000490.1
• NCBI GenBank Nucleotide #: NM_000499.3
• UniProt Accession #: P04798; Q53G18; A4F3V9; A4F3W0
• Molecular Weight: 31.3kDa
• NCBI Official Full Name: cytochrome P450 1A1
• NCBI Official Synonym Full Names: cytochrome P450, family 1, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP1A1
• NCBI Official Synonym Symbols: AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX
• NCBI Protein Information: cytochrome P450 1A1; CYPIA1; cytochrome P450-C; cytochrome P450-P1; cytochrome P450 form 6; xenobiotic monooxygenase; aryl hydrocarbon hydroxylase; flavoprotein-linked monooxygenase; cytochrome P1-450, dioxin-inducible; cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1
• UniProt Protein Name: Cytochrome P450 1A1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP1A1
• UniProt Entry Name: CP1A1_HUMAN

NCBI Description

This gene, CYP1A1, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member, CYP1A2, is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP1A1: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.14.1; Amino Acid Metabolism - tryptophan; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: endoplasmic reticulum membrane; mitochondrion

Molecular Function: flavonoid 3'-monooxygenase activity; oxidoreductase activity, acting on diphenols and related substances as donors; enzyme binding; iron ion binding; heme binding; steroid hydroxylase activity; oxidoreductase activity; oxygen binding; demethylase activity

Biological Process: coumarin metabolic process; response to nematode; response to arsenic; response to lipopolysaccharide; cellular lipid metabolic process; response to vitamin A; response to antibiotic; embryonic development ending in birth or egg hatching; flavonoid metabolic process; porphyrin metabolic process; response to wounding; arachidonic acid metabolic process; drug metabolic process; aging; response to food; response to drug; insecticide metabolic process; camera-type eye development; response to iron(III) ion; response to virus; epoxygenase P450 pathway; response to herbicide; vitamin D metabolic process; dibenzo-p-dioxin catabolic process; cell proliferation; response to hyperoxia; gut development; xenobiotic metabolic process; maternal process involved in parturition; 9-cis-retinoic acid biosynthetic process; response to hypoxia; amine metabolic process; hydrogen peroxide biosynthetic process