Fish cytochrome P450, family 1, subfamily A, polypeptide 1 ELISA Kit | CYP1A1 elisa kit

Fish Cytochrome P450 1A1, CYP1A1 ELISA Kit

Cat. Num. AAA706360

• Reactivity: Fish
• Synonyms: cytochrome P450; family 1; subfamily A; polypeptide 1; Fish Cytochrome P450 1A1; CYP1A1 ELISA Kit; Fish Cytochrome P450 1A1 (CYP1A1) ELISA kit; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX; OTTHUMP00000183469; aryl hydrocarbon hydroxylase; cytochrome P1-450; dioxin-inducible; cytochrome P450 1A1; subfamily I (aromatic compound; CYP1A1 elisa kit

• Reactivity: Fish
• Specificity: This assay has high sensitivity and excellent specificity for detection of fish CYP1A1. No significant cross-reactivity or interference between fish CYP1A1 and analogues was observed.
• Sequence Length: 367

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 75 pg/ml -1200 pg/ml.
• Sensitivity: The minimum detectable dose of fish CYP1A1 is typically less than 37.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest fish CYP1A1 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP1A1 elisa kit

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP1A1. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated CYP1A1. A competitive inhibition reaction is launched between CYP1A1 (Standards or samples) and Biotin-conjugated CYP1A1 with the pre-coated antibody specific for CYP1A1. The more amount of CYP1A1 in samples, the less antibody bound by Biotin-conjugated CYP1A1. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of CYP1A1 in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 194018576
• NCBI GeneID: 100170113
• NCBI Accession #: NP_001123377.1
• NCBI GenBank Nucleotide #: NM_001129905.1
• UniProt Accession #: P56591
• Molecular Weight: 59,230 Da
• NCBI Official Full Name: cytochrome P450 1A1
• NCBI Official Symbol: CYP1A1
• NCBI Protein Information: cytochrome P450 1A1; CYPIA1; cytochrome P450-C
• UniProt Protein Name: Cytochrome P450 1A1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP1A1
• UniProt Entry Name: CP1A1_SHEEP

Uniprot Description

Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor: Heme group