CYP19A1 recombinant protein

Recombinant Human CYP19A1 protein

Cat. Num. AAA1561720

• Gene Names: CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CYP19A1; Recombinant Human CYP19A1 protein; Aromatase; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; Estrogen synthase; CYP19A1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human CYP19A1 (Leu196-Val373) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for CYP19A1 recombinant protein

A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3, 17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1, 10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

Product Categories/Family for CYP19A1 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 13904858
• NCBI GeneID: 1588
• NCBI Accession #: NP_000094.2
• NCBI GenBank Nucleotide #: NM_000103.3
• UniProt Accession #: P11511; Q16731; Q3B764; Q58FA0
• Molecular Weight: 57,883 Da
• NCBI Official Full Name: cytochrome P450 19A1
• NCBI Official Synonym Full Names: cytochrome P450, family 19, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP19A1
• NCBI Official Synonym Symbols: ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• NCBI Protein Information: cytochrome P450 19A1; aromatase; estrogen synthase; estrogen synthetase; cytochrome P-450AROM; microsomal monooxygenase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily XIX (aromatization of androgens)
• UniProt Protein Name: Cytochrome P450 19A1
• Protein Family: Aromatase
• UniProt Gene Name: CYP19A1
• UniProt Synonym Gene Names: ARO1; CYAR; CYP19
• UniProt Entry Name: CP19A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. The gene expresses two transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; EC 1.14.14.14; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum

Molecular Function: electron carrier activity; iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; androgen metabolic process; steroid biosynthetic process; estrogen biosynthetic process; sterol metabolic process

Disease: Aromatase Deficiency; Aromatase Excess Syndrome