ENA-78 (CXCL5) Recombinant Protein | CXCL5 recombinant protein

Human ENA-78 (CXCL5) Recombinant

Cat. Num. AAA553205

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: ENA-78 (CXCL5); Human ENA-78 (CXCL5) Recombinant; ena-78; ena78; cxcl5; cxcl-5; ENA78; SCYB5; CXCL5 recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant ENA-78 was lyophilized from a 0.2 um filtered 20mM PB,100mM NaCl solution pH 7.5.
• Sequence Length: 114

• Host Note: Optimized DNA sequence encoding Human ENA-78 (CXCL5) mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Human ENA-78, generated by the proteolytic removal of the signal peptide; and propeptide and has a calculated molecular mass of approximately 8 kDa.; ; Recombinant CXCL5 is a disulfide-linked monomeric protein consisting of 75 amino acid residue subunits and migrates as an approximately 8 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for CXCL5 recombinant protein

Epithelial neutrophil- activating protein 78 (ENA-78) is a member of the CXC chemokines and acts as a potent chemoattractant and activator of neutrophil function. On stimulation in vitro, ENA-78 is highly expressed in many cell types. ENA-78 protein levels are strongly elevated in synovial fluid and blood of patients with rheumatoid arthritis. By in situ hybridization and immunofluorescence staining, ENA-78 has been recognized as a major CXC chemokine expressed in epithelial cells of the intestinal mucosa of patients with Crohn's disease, ulcerative colitis, and acute appendicitis.

Product Categories/Family for CXCL5 recombinant protein

Cytokines

References

• CXCL5 mediates uvb irradiation–induced pain .Science Translational Medicine, Jul 2011; 3: 90ra60.
• il-17a and tnf- exert synergistic effects on expression of CXCL5 by alveolar type ii cells in vivo and in vitro .J. Immunol., Mar 2011; 186: 3197 - 3205.
• evaluation of serum CXCL5 as a serum marker for prognosis of colorectal cancer patients. .ASCO Meeting Abstracts, Feb 2011; 29: 442.
• cxcl1 regulates pulmonary host defense to klebsiella infection via cxcl2, CXCL5, nf-b, and mapks .J. Immunol., Nov 2010; 185: 6214 - 6225.
• posttranslational modification of the nh2-terminal region of CXCL5 by proteases or peptidylarginine deiminases (pad) differently affects its biological activity .J. Biol. Chem., Sep 2010; 285: 29750 - 29759.