GRO-gamma (CXCL3) Recombinant Protein | GRO-gamma recombinant protein

Human GRO-gamma (CXCL3) Recombinant

Cat. Num. AAA553210

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: GRO-gamma (CXCL3); Human GRO-gamma (CXCL3) Recombinant; GRO-gamma; GROg; cxcl3; mip2; mip-2; GRO-gamma recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant GRO-gamma/CXCL3 was lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in 40mM NaCl, 10mM PB, pH 7.0.
• Sequence: MAHATLSAAP SNPRLLRVAL LLLLLVAASR RAAGASVVTE LRCQCLQTLQ GIHLKNIQSV NVRSPGPHCA QTEVIATLKN GKKACLNPAS PMVQKIIEKI LNKGSTN
• Sequence Length: 107

• Domain: IL8
• Host Note: Optimized DNA sequence encoding Human GRO-gamma mature chain was expressed in Escherichia Coli
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Molecular Weight Note: Native human GRO-gamma is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa.; ; Recombinant GRO-gamma is a disulfide-linked homodimeric protein consisting of 74 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for GRO-gamma recombinant protein

Growth regulated oncogene- gamma belongs to the family of chemotyctic cytokines called chemokines. It is identical with MGSA (melanoma growth stimulatory activity) and the new designation is CXCL3. This factor is known mainly because of its chemotactic activity. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

Product Categories/Family for GRO-gamma recombinant protein

Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 54144650
• NCBI GeneID: 2921
• NCBI Accession #: NP_002081.2
• NCBI GenBank Nucleotide #: NM_002090.2
• UniProt Accession #: P19876; Q4W5H9; Q6LD32
• Molecular Weight: 11,342 Da
• NCBI Official Full Name: C-X-C motif chemokine 3
• UniProt Protein Name: C-X-C motif chemokine 3
• UniProt Gene Name: CXCL3
• UniProt Synonym Gene Names: GRO3; GROG; SCYB3; GRO-gamma; MIP2-beta
• UniProt Entry Name: CXCL3_HUMAN

NCBI Description

This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL3: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: neutrophil chemotaxis; G-protein coupled receptor protein signaling pathway; immune response; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; inflammatory response; regulation of cell proliferation